Anti-Parvalbumin/PVALB Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P20472

Core Product Specifications and Parameters

Biological Background: Parvalbumin/PVALB Function and Localization

• Parvalbumin alpha is a calcium-binding protein that facilitates muscle relaxation by buffering intracellular calcium after contraction (By similarity).
• It binds two calcium ions with high affinity, serving as a slow calcium buffer in fast-twitch muscle fibers (PubMed:15122922, PubMed:39584689).
  Related references: PMID:15122922, PMID:39584689
• The protein undergoes post-translational modifications including acetylation and phosphorylation, as identified by proteomic studies.
• Structural analyses have resolved the three-dimensional conformation of parvalbumin, revealing its calcium-coordination mechanism.
• Encoded by the PVALB gene, the protein has a calculated molecular weight of 12 kDa and is also designated as D22S749.

Experimental Guidance and Technical Tips

• Since the immunogen comprises full-length human PVALB (amino acids 1-180), the antibody is expected to recognize both native and denatured protein. In Western blotting, a specific band at ~12 kDa should be observed.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), skeletal muscle tissue is recommended as a positive control, given high parvalbumin expression in fast-twitch fibers.
• In ELISA applications, consider using recombinant human PVALB protein or cell lysates as target antigen and validate the antibody’s performance under your assay conditions.
• The antibody is reported to cross-react with mouse and rat PVALB; however, confirm reactivity in your specific sample matrix and fixative conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, supporting diagnostic manufacturers with validated antibodies, antigen standards, and ancillary reagents. For researchers investigating calcium-signaling and muscle biology, we offer this high-quality anti-Parvalbumin monoclonal antibody alongside bulk supplies and custom sourcing services. Trust CamelBio to deliver reliable raw materials from concept to clinical application.