Anti-Parvalbumin (PVALB) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P20472

Core Product Specifications and Parameters

Biological Background: Parvalbumin Alpha Function and Localization

• Encoded by the PVALB gene, parvalbumin alpha is a ~12 kDa calcium-binding protein belonging to the EF-hand superfamily.
• In muscle, parvalbumin is thought to be involved in relaxation after contraction.
• It binds two calcium ions per molecule. Related references: PMID:15122922, PMID:39584689
• Key post-translational modifications include acetylation and phosphorylation.
• Associated keywords: metal-binding, muscle protein, calcium, 3D-structure, direct protein sequencing, repeat, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-110), which is highly conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blot (WB), expect a specific band at approximately 12 kDa in relevant muscle tissue or cell lysates; consider using appropriate loading controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate staining in muscle-containing tissue sections and optimize antigen retrieval conditions as needed.
• Determine the optimal working dilution empirically for each application; typical starting points may be 1:500–1:2000 for WB and 1:50–1:200 for IHC/IF.
• ELISA performance can be assessed using recombinant parvalbumin or tissue extracts; include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your assay development. This anti-PVALB polyclonal antibody is well-suited for muscle physiology, calcium signaling, and neuromuscular disease research. Through our integrated supply network, we can also source rare-target raw materials and provide technical consulting to accelerate your project from concept to clinic.