Anti-PARS2 Polyclonal Antibody for WB and ELISA - Q7L3T8

Core Product Specifications and Parameters

Biological Background: Prolyl-tRNA synthetase 2, mitochondrial Function and Localization

• PARS2 (probable proline--tRNA ligase, mitochondrial) is a member of the class II aminoacyl-tRNA synthetase family, also known as prolyl-tRNA synthetase (ProRS) or prolyl-tRNA synthetase 2.
• Catalyzes the ATP-dependent attachment of proline to its cognate tRNA(Pro) in a two-step reaction, essential for mitochondrial protein synthesis.
• Encoded by the PARS2 gene, this enzyme is critical for accurate mitochondrial translation and proper respiratory chain function.
• Localizes to the mitochondrial matrix, consistent with its role in mitochondrial gene expression.
• Annotated with functional keywords including ATP-binding, nucleotide-binding, ligase, and mitochondrial transit peptide; belongs to the class-II aminoacyl-tRNA synthetase family.
• Mutations in PARS2 are linked to developmental and epileptic encephalopathy (DEE75, EIEE75), underscoring its importance in neurodevelopment and mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 30–220), which includes conserved catalytic domains; use this information for epitope mapping or competitive studies.
• For Western blot, the predicted molecular weight is ~53 kDa; consider using mitochondrial-enriched samples and validate detection in your specific tissue or cell lysates.
• Apply the antibody in ELISA as a capture or detection reagent; optimal dilutions should be empirically determined.
• Demonstrates cross-reactivity with rat, indicating epitope conservation; confirm cross-reactivity in your experimental model.
• As a polyclonal antibody, it may recognize multiple epitopes or isoforms; verify specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop partner for IVD raw materials, supplying validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For mitochondrial targets like PARS2, we facilitate sourcing of rare raw materials and provide technical consulting to accelerate diagnostic development from concept to clinic. Let CamelBio bridge your research with reliable, high-quality immunoreagents.