Anti-PARP8 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N3A8

Core Product Specifications and Parameters

Biological Background: PARP8 Function and Localization

• PARP8, also known as ADP-ribosyltransferase diphtheria toxin-like 16 (ARTD16) or pART16, is a member of the poly(ADP-ribose) polymerase (PARP) superfamily.
• It functions as a mono-ADP-ribosyltransferase, catalyzing the transfer of a single ADP-ribose moiety from NAD+ onto specific target proteins, a post-translational modification involved in various cellular processes.
• PARP8 belongs to the ARTD subfamily, which includes enzymes with diphtheria toxin-like ADP-ribosyltransferase domains, and is classified under glycosyltransferase and transferase families.
• The PARP8 gene undergoes alternative splicing, generating multiple protein isoforms that may have distinct functional roles or tissue distributions.
• UniProt keywords associate PARP8 with ADP-ribosylation, NAD metabolism, and proteomics identification, highlighting its role in cellular signaling networks.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–300) of human PARP8; this region may be conserved across isoforms, so the antibody could detect multiple splice variants if they share this domain.
• For Western blot (WB), the predicted molecular weight of full-length PARP8 is approximately 96 kDa; consider using appropriate SDS-PAGE conditions and loading controls.
• In immunohistochemistry (IHC) on paraffin-embedded sections, antigen retrieval and antibody optimization may be necessary; validate in relevant tissue samples known to express PARP8.
• This antibody cross-reacts with mouse and rat PARP8 (as indicated), enabling translational studies; verify species-specific reactivity in your model system.
• For ELISA applications, use the antibody as capture or detection reagent according to your assay format; titration and buffer conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers integrated access to IVD raw materials, including validated antibody pairs, bulk polyclonal antibodies, and recombinant proteins. Our supply chain covers PARP8 and related ADP-ribosylation targets, supporting assay development from discovery to clinical application. Contact us for customized sourcing of rare-target antibodies and ancillary reagents to accelerate your IVD pipeline.