Anti-PARP4 Polyclonal Antibody for WB, ELISA - Q9UKK3

Core Product Specifications and Parameters

Biological Background: PARP4 Function and Localization

• PARP4, also known as VPARP, ARTD4, or PH5P, is a member of the poly(ADP-ribose) polymerase family, specifically a mono-ADP-ribosyltransferase.
• It catalyzes the mono-ADP-ribosylation of target proteins, utilizing NAD as a substrate [PMID:25043379]. Related references: PMID:25043379
• PARP4 is a core component of the vault particle, a large ribonucleoprotein complex implicated in cargo transport and signal transduction [PMID:40691181]. Related references: PMID:40691181
• The protein localizes to multiple cellular compartments, including the cytoplasm, nucleus, and cytoskeleton (spindle apparatus).
• It is widely expressed across human tissues, with the highest levels observed in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes, and pancreas.
• Post-translational modifications include phosphorylation and methylation, as identified by proteomics and direct protein sequencing.
• The human PARP4 gene encodes a ~193 kDa protein (192.6 kDa calculated), and immunogen design often targets the N-terminal region (e.g., amino acids 1-300).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-300 amino acids of human PARP4; the antibody may detect full-length PARP4 and possibly N-terminal fragments. Validate in your sample system.
• Recommended applications include Western blot (WB) and ELISA. Optimize antibody dilution and incubation conditions for your specific assay and sample type.
• Cross-reactivity is reported for human, mouse, and rat; verify in your experimental context if using other species.
• The predicted molecular weight is ~193 kDa; use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• For vault complex studies, consider co-immunoprecipitation or immunofluorescence using this antibody in relevant cell lines or tissue samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents to support every stage of diagnostic development. This anti-PARP4 polyclonal antibody is ideal for research in vault biology, ADP-ribosylation, and cellular transport, and CamelBio can assist with rare target sourcing and custom material requirements. From concept to clinic, CamelBio provides integrated supply solutions to accelerate your assay development and production workflows.