Anti-PARP2 Rabbit Polyclonal Antibody for WB, ELISA - Q9UGN5

Core Product Specifications and Parameters

Biological Background: PARP2 Function and Localization

• PARP2 (Poly [ADP-ribose] polymerase 2), also known as ARTD2, ADPRT2, PARP-2, ADPRTL2, ADPRTL3, pADPRT-2, is a member of the poly(ADP-ribose) polymerase family.
• Mediates poly-ADP-ribosylation of proteins, a post-translational modification essential for DNA repair and genome stability. Related references: PMID:10364231, PMID:25043379, PMID:27471034
• Upon DNA damage, interaction with HPF1 switches PARP2 to serine-specific ADP-ribosylation of target proteins, such as histones, promoting chromatin decompaction and recruitment of repair factors. Related references: PMID:25043379, PMID:30104678, PMID:30321391, PMID:32939087, PMID:28190768, PMID:32028527, PMID:34108479
• HPF1 also restricts the polymerase activity of PARP2, limiting the length of poly-ADP-ribose chains. Related references: PMID:34732825, PMID:34795260
• Specifically catalyzes the formation of branched poly-ADP-ribose chains, which are recognized by reader proteins such as APLF. Related references: PMID:30104678
• In addition to protein substrates, PARP2 can ADP-ribosylate DNA at 5'-terminal phosphates in nicked duplexes. Related references: PMID:27471034, PMID:29361132
• Localizes to the nucleus and is associated with chromosomes.
• Widely expressed, with highest levels in brain, heart, pancreas, skeletal muscle, and testis. Related references: PMID:10364231
• Subject to acetylation and phosphorylation; functions as an allosteric enzyme activated by DNA damage.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (residues 450–550) of human PARP2; the antibody may recognize full-length PARP2 and splice variants containing this epitope.
• For Western blot, expect a major band near 66kDa in lysates from human, mouse, or rat cells/tissues; validate the antibody in your specific sample system.
• This polyclonal antibody is suitable for ELISA; optimize coating and detection conditions as needed.
• Monitor lot-to-lot consistency when using this polyclonal antibody in quantitative or diagnostic assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with one-stop access to IVD raw materials and technical support. This anti-PARP2 antibody supports DNA repair and ADP-ribosylation research, and we offer a full range of validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.