Anti-PARP12 Polyclonal Antibody for WB, ELISA - Q9H0J9

Core Product Specifications and Parameters

Biological Background: PARP12 Function and Localization

• PARP12 (Protein mono-ADP-ribosyltransferase PARP12) is a member of the poly(ADP-ribose) polymerase family, characterized by its zinc-finger CCCH domains and catalytic ADP-ribosyltransferase activity.
• It functions as a mono-ADP-ribosyltransferase, catalyzing the transfer of a single ADP-ribose moiety from NAD+ to target proteins, regulating diverse cellular processes (PubMed:25043379, PubMed:34969853).
  Related references: PMID:25043379, PMID:34969853
• PARP12 exerts antiviral activity by cooperating with PARP11 to restrict Zika virus replication (PubMed:34187568) and directly ADP-ribosylating alphaviral non-structural proteins nsP3 and nsP4 during IFN-γ immune activation (PubMed:39888989).
  Related references: PMID:34187568, PMID:39888989
• As a component of the PRKD1 regulatory cascade, PARP12 mono-ADP-ribosylates GOLGA1, selectively controlling a major branch of the basolateral transport pathway (PubMed:34969853).
  Related references: PMID:34969853
• It plays a key role in mitochondrial homeostasis by inhibiting PINK1/Parkin-dependent mitophagy and promoting cartilage degeneration through upregulation of ISG15 and ISGylation, which impairs ubiquitination and SUMOylation of mitofusins MFN1/2 (PubMed:39465252).
  Related references: PMID:39465252
• Subcellularly, PARP12 localizes to the nucleus, Golgi apparatus (trans-Golgi network), cytoplasm, and stress granules, reflecting its involvement in multiple signaling hubs.
• The protein features zinc-finger domains and undergoes phosphorylation, consistent with its classification as a phosphoprotein and zinc-binding transferase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 462-701 of human PARP12, targeting a central region; the predicted molecular weight is approximately 79 kDa. Confirm expression in your sample system by including appropriate positive and negative controls.
• For western blotting, optimize antibody dilution and detection conditions empirically using lysates from human, mouse, or rat cell lines known to express PARP12 (e.g., immune or stressed cells).
• In ELISA applications, consider using recombinant PARP12 protein as a standard to validate specificity and sensitivity; plate coating and blocking conditions should be optimized according to your laboratory protocol.
• Because PARP12 participates in dynamic cellular processes such as antiviral defense and stress response, treatment of cells with interferons or stress inducers may enhance target detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a one-stop sourcing bridge for IVD raw materials, including validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents. Our PARP12 antibody supports investigations into antiviral immunity, Golgi transport, and mitochondrial regulation, offering researchers reliable access to high-quality biological reagents. With extensive supply capabilities, we help streamline your development from concept to clinic, including rare target raw-material sourcing.