Anti-PARN Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95453

Core Product Specifications and Parameters

Biological Background: PARN Function and Localization

• PARN (Poly(A)-specific ribonuclease) is a 3'-exoribonuclease with strong preference for poly(A) tails of mRNAs, initiating deadenylation-dependent mRNA decay. Related references: PMID:10882133 PMID:11359775 PMID:12748283
• It interacts with both the 3'-poly(A) tail and the 5'-cap structure, a coupling essential for efficient deadenylation. Related references: PMID:10882133 PMID:11359775 PMID:12748283
• PARN participates in nonsense-mediated mRNA decay (NMD) and degradation of ARE-containing unstable mRNAs, possibly through interaction with KHSRP. Related references: PMID:10882133
• Beyond mRNAs, PARN can trim poly(A) tails of microRNAs (e.g., MIR21) and H/ACA box snoRNAs, influencing their stability. Related references: PMID:22442037 PMID:25049417
• The protein localizes to the nucleus, cytoplasm, and nucleolus, reflecting its multifaceted roles in RNA metabolism.
• PARN is ubiquitously expressed in human tissues and belongs to the deadenylating nuclease family; it requires magnesium as a cofactor.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing generates multiple isoforms.
• Mutations in PARN are associated with dyskeratosis congenita, linking it to telomere biology disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 280 amino acids; consider epitope accessibility in your assay design. For WB, a starting dilution range of 1:500–1:2000 with a suitable secondary antibody is suggested, with optimization based on your sample’s expression level.
• In IF/ICC, fixation and permeabilization conditions (e.g., methanol vs. PFA/Triton) can influence antigen detection; validate in your cell line of interest. PARN’s nuclear/cytoplasmic distribution may require careful co-staining controls.
• For ELISA, the polyclonal nature may enhance signal capture; consider pairing with a monoclonal antibody for a sandwich ELISA. Validate with recombinant protein or cell lysates expressing human PARN.
• The predicted MW is ~73 kDa; confirm by running appropriate molecular weight markers and consider potential isoform-dependent shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide comprehensive IVD raw-material solutions. This anti-PARN antibody supports studies in RNA biology, mRNA decay, and post-transcriptional regulation, complementing our portfolio of validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. From target discovery to clinical application, we facilitate timely access to reliable raw materials tailored to your assay development needs.