Anti-Parathyroid Hormone (PTH) Rabbit Polyclonal Antibody for WB, ELISA - P01270

Core Product Specifications and Parameters

Biological Background: Parathyroid Hormone (PTH) Function and Localization

• Parathyroid hormone (PTH) is a secreted hormone that elevates calcium level by dissolving bone salts and preventing renal calcium excretion, acting via the PTH1R receptor and G protein-coupled signaling (PubMed:11604398, PubMed:35932760). Related references: PMID:11604398 PMID:35932760
• PTH stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed:21076856). Related references: PMID:21076856
• Encoded by the PTH gene, parathyroid hormone is initially synthesized as a preprohormone and undergoes cleavage on basic residues to generate the mature 84-amino acid peptide (Keywords: Cleavage on pair of basic residues).
• The protein is secreted into the bloodstream to exert endocrine effects (Subcellular location: Secreted).
• Disease-associated variants in PTH are linked to calcium metabolism disorders such as hypoparathyroidism (Keyword: Disease variant).
• The PTH protein has been extensively characterized by 3D structure analysis, direct protein sequencing, and proteomics (Keywords: 3D-structure, Direct protein sequencing, Proteomics identification).

Experimental Guidance and Technical Tips

• For Western blotting, the antibody detects a band of approximately 13 kDa under reducing conditions; optimize protein loading and antibody dilution using positive controls such as recombinant PTH or parathyroid gland extracts.
• In ELISA, this antibody can serve as either capture or detection antibody; perform checkerboard titration with the antigen and pairing antibody to determine optimal working concentrations.
• The antibody shows cross-reactivity with mouse and rat PTH, suggesting recognition of a conserved epitope; validate in the target species using appropriate control lysates or recombinant proteins.
• Because PTH is a secreted peptide, consider using serum, plasma, or conditioned medium samples in addition to tissue lysates, and include protease inhibitors in the collection protocol.
• Confirm the antibody’s specificity by pre-adsorption with the immunogen or by using PTH knockout models if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a comprehensive range of IVD raw materials and technical support. This anti-PTH polyclonal antibody is sourced to support calcium metabolism research and assay development. In addition to this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic projects from concept to clinic.