Anti-Parathyroid Hormone (PTH) Monoclonal Antibody for WB, IF-P, ELISA - P01270

Core Product Specifications and Parameters

Biological Background: Parathyroid Hormone (PTH) Function and Localization

• Parathyroid hormone (PTH), also known as parathormone or parathyrin, is a secreted hormone critical for calcium homeostasis. It elevates blood calcium levels by promoting bone resorption and reducing renal calcium excretion. Related references: PMID:11604398, PMID:35932760
• PTH exerts its effects by binding to the PTH1R receptor, activating G protein-coupled receptor signaling pathways. Related references: PMID:18375760, PMID:35932760
• In osteoblastic cells, PTH stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis. Related references: PMID:21076856
• PTH is secreted from cells, consistent with its role as a circulating hormone. It contains a signal peptide for extracellular release.
• Additional UniProt keywords include cleavage on pair of basic residues, direct protein sequencing, disease variant, hormone, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• Immunogen Design: The antibody was raised against a synthetic peptide spanning amino acids 1-100, likely targeting the N-terminal region of PTH. This region may be important for receptor binding and biological activity.
• Western Blotting (WB): The predicted molecular weight of mature PTH is approximately 13 kDa. Use appropriate low-molecular-weight markers and optimize transfer conditions for small proteins.
• Immunofluorescence (IF-P): This antibody may be suitable for detecting PTH in parathyroid tissue or relevant cell models. Validate staining patterns with appropriate controls.
• ELISA: The monoclonal nature of this antibody makes it a potential candidate for sandwich ELISA development when paired with a suitable detection antibody. Confirm performance in your sample matrix.
• Cross-reactivity: Demonstrated reactivity with human, mouse, and rat PTH allows for potential use across these species. Verify in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio simplifies your procurement from discovery to diagnostics. This anti-PTH monoclonal antibody supports calcium metabolism and bone disease research, and we offer a full range of validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your assay development. Let CamelBio be your bridge from concept to clinic.