Anti-PAR2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55085

Core Product Specifications and Parameters

Biological Background: PAR2 Function and Localization

• PAR2, also known as F2RL1, GPR11, Coagulation factor II receptor-like 1, and Thrombin receptor-like 1, is a G protein-coupled receptor encoded by the F2RL1 gene.
• Functions as a receptor for trypsin and trypsin-like enzymes, activating downstream signaling pathways such as PLC, MAPK, I-kappaB kinase/NF-kappaB, and Rho. Related references: PMID:28445455
• Plays a key role in modulating inflammatory responses and innate/adaptive immunity, acting as a sensor for proteolytic enzymes during infection; can signal synergistically with TLR4 and modulate TLR3 signaling.
• Contributes to endothelial barrier integrity: mediating barrier protective signaling via coagulation factor Xa, and regulating barrier integrity during neutrophil extravasation via PRTN3 cleavage. Related references: PMID:22409427 PMID:23202369
• Proposed to have a bronchoprotective role in airway epithelium, but can also compromise the epithelial barrier by interrupting E-cadherin adhesion. Related references: PMID:10086357
• Associates with G proteins alpha subunits including GNAQ, GNA11, GNA12, GNA13, and can additionally signal through G(i) subunit alpha. Related references: PMID:21627585
• Subcellular localization is primarily at the cell membrane.
• Tissue expression: widely expressed, with highest levels in pancreas, liver, kidney, small intestine, and colon; not detected in brain or skeletal muscle. Related references: PMID:7556175 PMID:8615752

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 15–94) of human PAR2; the antibody may recognize epitopes within this domain. Consider validating cross-reactivity in your species of interest.
• For WB, a band around 44 kDa is expected. Take into account potential glycosylation that may shift the observed molecular weight.
• In IF/ICC, verify staining specificity using appropriate positive and negative control cell lines or knockdown models.
• For ELISA, the antibody can be tested as a capture or detection reagent in your assay system; optimal pairings should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from early development through clinical translation. For your GPCR and inflammatory signaling studies with this PAR2 antibody, we can supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents. Leverage our network to access rare-target raw materials and streamline your assay development pipeline.