Anti-PAP Rabbit Polyclonal Antibody for WB, ELISA - P51003

Core Product Specifications and Parameters

Biological Background: PAP Function and Localization

• Poly(A) polymerase alpha (PAPOLA), also known as polynucleotide adenylyltransferase alpha, is a key enzyme in mRNA processing.
• It catalyzes the addition of the 3'-poly(A) tail to nascent mRNA transcripts, essential for mRNA stability, export, and translation.
• PAPOLA is also required for the endoribonucleolytic cleavage reaction at certain polyadenylation sites, acting in concert with the cleavage and polyadenylation specificity factor (CPSF).
• The enzyme localizes to both the cytoplasm and nucleus, consistent with its role in mRNA processing.
• PAPOLA is an ATP-binding protein that requires divalent metal ions such as magnesium or manganese for its catalytic activity.
• Post-translational modifications include acetylation and phosphorylation, and it is subject to alternative splicing and Ubl conjugation (isopeptide bond).
• It contains nucleotide-binding and RNA-binding domains, and serves as a transferase, specifically a nucleotidyltransferase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 605-745), which includes the CPSF interaction domain; the polyclonal nature may allow recognition of multiple epitopes.
• For Western blot, the predicted molecular weight is approximately 83 kDa; ensure adequate separation and transfer conditions, and validate with appropriate human cell lysates.
• ELISA applications may benefit from optimizing coating conditions and considering paired antibody formats; verify performance in your specific assay buffer system.
• Cross-reactivity is reported for human; if working with other species, conduct pilot experiments to confirm reactivity.
• Due to alternative splicing, additional bands may be observed; confirm band identity with relevant controls or knockdown/knockout models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with critical reagents like this anti-PAP polyclonal antibody for mRNA polyadenylation studies. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and access to rare target raw materials, supporting seamless development from concept to clinic.