Anti-Pannexin-1 Polyclonal Antibody for WB, ELISA - Q96RD7

Core Product Specifications and Parameters

Biological Background: Pannexin-1 Function and Localization

• Pannexin-1 (PANX1) is an integral membrane protein and a member of the pannexin family, forming hexameric channels at the plasma membrane and endoplasmic reticulum.
• Functions as an anion-selective channel with relatively low conductance, playing key roles in blood pressure regulation, apoptotic cell clearance, and oogenesis. Related references: PMID:15304325 PMID:16908669 PMID:20829356
• Upon phosphorylation or C-terminal cleavage, mediates ATP and UTP release, acting as a large-pore channel that facilitates communication and phagocyte attraction during apoptosis. Related references: PMID:32238926
• May function as a Ca(2+)-leak channel in the endoplasmic reticulum membrane, contributing to ER calcium homeostasis. Related references: PMID:16908669
• Widely expressed with highest levels in oocytes and brain, and low levels in sperm cells. Related references: PMID:30918116
• Subject to alternative splicing and post-translational modifications including phosphorylation, S-nitrosylation, and N-glycosylation.
• Disease-associated aliases (OOMD7, OZEMA7) link PANX1 to oculodentodigital dysplasia and related disorders.
• Contains four transmembrane helices with disulfide bonds stabilizing structure; predicted molecular weight ~48 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 50-150 of human Pannexin-1 (NP_056183.2). This region may include an extracellular or intracellular loop; consider optimization of antibody incubation conditions for native vs. denatured protein detection.
• For Western blot, consider a starting dilution of 1:500-1:2000, and validate with appropriate positive and negative controls in your sample system.
• For ELISA, this antibody can be used for direct antigen detection or as part of a sandwich pair; we recommend cross-testing with a matched detection antibody for optimal performance.
• Note that Pannexin-1 is expressed in many tissues and may undergo cleavage-induced activation in apoptotic samples; take precautions to minimize post-lysis modifications if studying channel function.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research institutes with validated antibodies, bulk reagents, and specialized targets like Pannexin-1. Our streamlined supply chain supports your work from concept to clinic, particularly in ion channel biology and apoptosis research. Let us help you bridge the gap between discovery and diagnostic application.