Anti-PANK2 Rabbit Polyclonal Antibody for WB and ELISA - Q9BZ23

Core Product Specifications and Parameters

Biological Background: Pantothenate kinase 2, mitochondrial Function and Localization

• Pantothenate kinase 2, mitochondrial (PANK2) catalyzes the first and rate-limiting step in coenzyme A (CoA) biosynthesis: the ATP-dependent phosphorylation of pantothenate to 4'-phosphopantothenate. Related references: PMID:15659606, PMID:16272150, PMID:17242360
• Alternative splicing and initiation produce both a mitochondrial isoform (containing a transit peptide) and a cytoplasmic isoform; the mitochondrial form additionally localizes to the nucleus.
• PANK2 is required for the angiogenic activity of human umbilical vein endothelial cells (HUVEC). Related references: PMID:30221726
• The protein is ubiquitously expressed with highest levels in testis and brain, consistent with its role in CoA-dependent metabolic and neurological processes.
• Mutations in PANK2 are linked to pantothenate kinase-associated neurodegeneration (PKAN), a subtype of neurodegeneration with brain iron accumulation (NBIA).
• Classified as a kinase, transferase, and phosphoprotein, PANK2 is an ATP-binding enzyme central to cellular metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 300-400) of human PANK2; verify target detection in the relevant species (human, mouse) and sample type.
• For Western blot, the predicted molecular weight is 63 kDa; consider using reducing conditions and include appropriate positive controls (e.g., HeLa, HEK293, or mouse testis lysates).
• Cross-reactivity with mouse is reported; validate specificity in mouse models if applicable.
• For ELISA, use recombinant protein or peptide to confirm antibody binding and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, including antibodies against key neurological targets such as PANK2. Our extensive portfolio features validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents—designed to accelerate your assay development from concept to clinic. Whether you need established reagents or rare-target sourcing, CamelBio offers integrated supply solutions to support your neurodegeneration and metabolism research programs.