Anti-pan-Trk Rabbit Monoclonal Antibody for WB, ELISA - P04629 / Q16620 / Q16288

Core Product Specifications and Parameters

Biological Background: pan-Trk (TrkA/TrkB/TrkC) Function and Localization

• Pan-Trk antibody recognizes the three members of the tropomyosin receptor kinase family: TrkA (encoded by NTRK1), TrkB (NTRK2), and TrkC (NTRK3), which serve as receptors for neurotrophins.
• TrkA is a high-affinity receptor for nerve growth factor (NGF) and plays a crucial role in the development, differentiation, and survival of sympathetic and sensory neurons. Upon NGF binding, TrkA homodimerizes and autophosphorylates, initiating intracellular signaling. Related references: PMID:1281417, PMID:15488758, PMID:17196528
• Through phosphorylation of adaptors like SHC1 and FRS2, TrkA activates the GRB2-Ras-MAPK and PI3K-AKT1 pathways, which govern cell growth, differentiation, and survival.
• TrkA localizes to the cell membrane and, upon ligand-mediated internalization, traffics through early, late, and recycling endosomes.
• Alternative splicing generates several isoforms: TrkA-I is widely expressed in non-neuronal tissues, TrkA-II is neuronal-specific, and TrkA-III (expressed in neural stem/progenitor cells) can exhibit oncogenic activity and promote angiogenesis.
• Chromosomal rearrangements involving NTRK1 result in oncogenic fusions, highlighting its role as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human pan-Trk, suggesting recognition of a conserved epitope across TrkA/B/C. Validate reactivity in your specific sample system and consider optimizing antibody dilution for WB or ELISA.
• For western blotting, expect bands at ~140 kDa (TrkA), ~145 kDa (TrkB), and ~145 kDa (TrkC) based on published molecular weights; however, experimental conditions may shift migration.
• The antibody is recommended for human, mouse, and rat samples. Cross-reactivity with other species should be independently verified.
• Due to the expression of truncated or oncogenic isoforms, additional bands may be observed. Confirm band identity using appropriate controls or knockdown/knockout models.

CamelBio: Your One-Stop Sourcing Bridge

• As a specialized provider of IVD raw materials, CamelBio offers a comprehensive portfolio that includes validated antibody pairs, recombinant monoclonal antibodies, and bulk ancillary reagents—supporting your transition from concept to clinic.
• Our sourcing network helps diagnostic manufacturers and research laboratories secure reliable pan-Trk and other neurotrophin pathway reagents, simplifying supply chain complexities for rare or multi-target proteins.
• Whether you need optimized antibodies for WB/ELISA or seek custom bulk orders, CamelBio serves as your strategic sourcing partner, ensuring quality and consistency for neuroscience research and assay development.