Anti-Pan Cadherin Polyclonal Antibody for WB, IP, ELISA - P12830 / P19022 / P22223 / P55283

Core Product Specifications and Parameters

Biological Background: Pan Cadherin Function and Localization

• Cadherins are a family of calcium-dependent cell adhesion molecules that mediate homophilic cell-cell interactions, crucial for tissue organization and morphogenesis.
• The immunogen corresponds to a conserved region within the cytoplasmic domain of cadherins, suggesting reactivity with multiple cadherin family members (e.g., CDH1, CDH2, CDH3, CDH4).
• E-cadherin (CDH1), a major epithelial cadherin, functions as a tumor suppressor and is involved in epithelial cell adhesion, migration, and proliferation. Related references: PMID:11976333
• CDH1 engages in calcium-dependent homophilic binding that promotes the formation of adherens junctions and regulates actin cytoskeleton organization via interactions with proteins like AMOTL2.
• CDH1 also plays a role in desmosome assembly by recruiting desmosomal cadherins DSG2 and DSP to cell-cell contacts. Related references: PMID:29999492
• Subcellularly, cadherins localize to adherens junctions, desmosomes, the cell membrane, and can be found in endosomes and Golgi, reflecting dynamic trafficking.
• CDH1 is prominently expressed in epithelial tissues, including skin, liver, and esophageal epithelium, and is detected in macrophages at protein level.
• Knockout or mutation of CDH1 is linked to hereditary diffuse gastric cancer and ectodermal dysplasia, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from a conserved intracellular region (aa 800-882) of human pan-cadherin; this design may favor recognition of multiple cadherins across species. Validate reactivity in your specific model system.
• For Western blot (WB), consider using a denaturing/reducing protocol as the epitope is linear. Expect multiple bands corresponding to different cadherin isoforms (e.g., ~120-130 kDa for classical cadherins). Optimize blocking and antibody concentration for each cell type.
• In immunoprecipitation (IP), the antibody may pull down full-length cadherins and associated catenins; include appropriate controls to distinguish specific interactions.
• For ELISA, coat plates with peptide or tissue lysates; cross-titrate the antibody and detection reagent to establish working dilutions.
• Given the reported cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models; however, always verify by positive and negative control lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers from concept to clinic. This pan-cadherin antibody is part of our extensive portfolio for cell adhesion and cancer research, and we offer validated antibody pairs, recombinant proteins, and ancillary reagents to streamline your assay development. For rare or custom targets, our sourcing capabilities can help you secure the raw materials you need.