Anti-pan 14-3-3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P27348 / P31946 / P61981 / P62258 / P63104 / Q04917 / P31947

Core Product Specifications and Parameters

Biological Background: pan 14-3-3 Function and Localization

• The 14-3-3 protein family in humans consists of seven isoforms—beta, gamma, epsilon, eta, sigma, theta, and zeta—encoded by genes YWHAB, YWHAG, YWHAE, YWHAH, SFN, YWHAQ, and YWHAZ, respectively, and are recognized by this pan-specific antibody.
• These adapter proteins bind phosphoserine/phosphothreonine motifs on a wide array of signaling molecules, thereby modulating kinase activities (e.g., negative regulation of PDPK1), apoptosis, and metabolic pathways.
• The theta isoform (P27348), also known as 14-3-3 protein tau or HS1, is abundantly expressed in brain, heart, and pancreas, with lower levels in kidney and placenta; its expression is upregulated in amyotrophic lateral sclerosis (ALS) spinal cord.
• 14-3-3 proteins localize predominantly to the cytoplasm, consistent with their role in transducing signals from the cell membrane to intracellular effectors.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), are critical for regulating 14-3-3 dimerization, stability, and interaction with partners.
• Keywords from curated databases: 3D-structure, Acetylation, Cytoplasm, Direct protein sequencing, Isopeptide bond, Nitration, Phosphoprotein, Proteomics identification, Reference proteome, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the highly conserved N-terminal region of 14-3-3 theta (NP_006817.1, amino acids 1-100), ensuring broad recognition of all seven human isoforms; cross-species reactivity in mouse and rat has been confirmed.
• For Western blot, multiple bands between 27 and 29 kDa are expected due to isoform diversity; include appropriate positive controls (e.g., HeLa, HEK293, or brain tissue lysates) and validate band patterns with known expression profiles.
• In immunofluorescence/immunocytochemistry (IF/ICC), permeabilization is required as the target is cytoplasmic; expect diffuse staining, which may intensify or re-localize in response to specific stimuli.
• For ELISA applications, the antibody can serve as a capture or detection reagent, but optimal coating concentration and assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. The pan 14-3-3 antibody supports research in cell signaling and disease biomarker discovery. Leverage CamelBio's consulting and technical services to streamline your diagnostic development from concept to clinical application.