Anti-PAM16 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y3D7

Core Product Specifications and Parameters

Biological Background: PAM16 Function and Localization

• PAM16 (Presequence translocated-associated motor subunit 16) is also known as TIM16, MAGMAS, TIMM16, and CGI-136. It is a component of the TIM23/PAM translocase complex responsible for importing nuclear-encoded proteins into the mitochondrial matrix.
• The protein functions as a key regulator of ATP-dependent protein translocation, modulating the import motor associated with the TIM23 translocon.
• PAM16 directly inhibits DNAJC19, preventing excessive stimulation of the mitochondrial chaperone HSPA9/Mortalin ATPase activity, thus controlling the import cycle.
• It is an integral inner mitochondrial membrane protein, anchored to the mitochondrial inner membrane.
• PAM16 is ubiquitously expressed across tissues, consistent with its fundamental role in mitochondrial biogenesis and protein homeostasis.
• UniProt annotations highlight its involvement in disease variant (associated with dwarfism), its status as a phosphoprotein, and its classification as a reference proteome component.
• The predicted molecular weight is approximately 13.8 kDa, consistent with the observed 14 kDa band in western blotting.

Experimental Guidance and Technical Tips

• Given the immunogen covers amino acids 21-125, which excludes the N-terminal mitochondrial targeting sequence, the antibody likely recognizes the mature form. For WB, expect a band near 14 kDa; consider using mitochondrial fraction enrichment for enhanced signal.
• When performing ELISA, use appropriate coating buffers and validate with recombinant protein or cell lysates; this polyclonal may be suitable as both capture and detection antibody.
• Cross-reactivity with human and mouse is indicated; validate in your specific sample system (e.g., HeLa, HEK293, or mouse tissues).
• Since PAM16 is a mitochondrial protein, ensure lysis conditions are compatible with membrane protein extraction, such as using RIPA buffer or specialized mitochondrial isolation kits.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized rare-target raw-material procurement. For researchers studying mitochondrial protein import and associated disorders, this anti-PAM16 antibody complements our portfolio to support diagnostic and research workflows from concept to clinic.