Anti-Palladin Rabbit mAb for WB, ELISA - Q8WX93

Core Product Specifications and Parameters

Biological Background: Palladin Function and Localization

• Palladin is a cytoskeletal protein essential for organizing the actin cytoskeleton, influencing cell morphology, motility, adhesion, and cell-extracellular matrix interactions (FUNCTION).
• It may serve as a scaffolding molecule that modulates actin polymerization and the assembly of actin filaments into higher-order structures, interacting with both monomeric and filamentous actin-binding proteins (FUNCTION).
• Palladin localizes to dynamic actin-rich sites, including lamellipodia, membrane ruffles, podosomes, focal adhesions, sarcomeric Z-lines, and neuronal growth cones (SUBCELLULAR LOCATION).
• The protein is expressed in both muscle and non-muscle tissues, with high levels in prostate, ovary, colon, and kidney, but low or absent in spleen, skeletal muscle, lung, and peripheral blood lymphocytes (TISSUE SPECIFICITY).
• Overexpression has been observed in several sporadic pancreatic cancer cell lines (TISSUE SPECIFICITY).
• Alternative splicing generates multiple isoforms, which may have functional differences (FUNCTION; KEYWORDS: Alternative splicing).
• Post-translational modifications include phosphorylation, and the presence of immunoglobulin-like domains and disulfide bonds (KEYWORDS: Phosphoprotein, Disulfide bond, Immunoglobulin domain, Repeat).
• Palladin is also involved in targeting alpha-actinin (ACTN) to specific subcellular foci and plays a role in dendritic cell maturation (FUNCTION).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-100 of human Palladin (NP_001159582.1); verify reactivity with your isoform(s) of interest, as alternative splicing may produce variants lacking this N-terminal region.
• In Western blotting, expect a band around 150-151 kDa; molecular weight may shift due to phosphorylation and isoform diversity. Validate using a palladin-positive cell lysate.
• This antibody is recommended for ELISA; optimize coating conditions and detection with an appropriate anti-rabbit secondary antibody.
• Cross-reactivity has been confirmed for human samples; for other species, perform empirical validation.
• If adapting for immunocytochemistry, consider fixation and permeabilization protocols that preserve cytoskeletal structures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of IVD raw materials, including validated monoclonal antibodies like this anti-Palladin clone. Whether you require antibody pairs for ELISA development, bulk production-grade antibodies, or ancillary reagents for cytoskeleton and oncology research, our integrated supply chain supports your development pathway from concept to clinic.