Anti-PALB2 Rabbit Polyclonal Antibody for WB and ELISA - Q86YC2

Core Product Specifications and Parameters

Biological Background: PALB2 Function and Localization

• PALB2 (Partner and Localizer of BRCA2) is a nuclear protein that plays a critical role in homologous recombination repair (HRR) by recruiting BRCA2 and RAD51 to DNA double-strand breaks. Related references: PMID:16793542 PMID:19369211 PMID:19423707
• PALB2 strongly stimulates RAD51 DNA strand invasion activity, stabilizes the RAD51 nucleoprotein filament against disruptive factors, and helps overcome replication protein A (RPA) inhibition. Related references: PMID:20871615
• PALB2 functionally cooperates with RAD51AP1 to promote D-loop formation by RAD51, an essential step in homologous recombination. Related references: PMID:20871616
• PALB2 serves as a molecular scaffold for the BRCA1-PALB2-BRCA2 complex, which is essential for HRR, and also scaffolds a complex containing RAD51C and BRCA2 via its WD repeats. Related references: PMID:19369211 PMID:24141787
• PALB2 promotes the localization and stability of BRCA2, protecting it from proteasome-mediated degradation, and enables its recombinational repair and checkpoint functions. Related references: PMID:16793542
• PALB2 binds DNA with high affinity for D-loop structures and may play a role in the extension step after strand invasion, cooperating with BRCA2 in POLH localization and DNA polymerization at collapsed replication forks. Related references: PMID:20871616 PMID:24485656
• PALB2 is localized in the nucleus, consistent with its role in DNA repair.
• PALB2 is classified as a tumor suppressor and is associated with Fanconi anemia; it undergoes phosphorylation and contains WD repeats.

Experimental Guidance and Technical Tips

• The immunogen (aa 99–198) lies within the N-terminal region of PALB2; consider this epitope location when interpreting results in protein-interaction studies.
• For Western blot, the predicted molecular weight is ~131 kDa. Use a low-percentage gel (e.g., 6–8%) and optimize transfer conditions for high-molecular-weight proteins.
• PALB2 may be prone to degradation; use fresh lysates with protease inhibitors and avoid multiple freeze-thaw cycles.
• As a polyclonal antibody, confirm lot-to-lot consistency by testing on a known positive control.
• Validate antibody specificity in your experimental system (e.g., PALB2 knockdown or knockout cell lines) before large-scale experiments.

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CamelBio provides diagnostic manufacturers and research institutions with one-stop procurement of validated IVD raw materials, including antibodies like this PALB2 polyclonal antibody. We offer comprehensive sourcing for DNA repair and oncology research, from monoclonal/polyclonal antibodies and validated antibody pairs to ancillary reagents and rare biomarker targets. Our supply network supports your development from concept to clinical application with reliable bulk raw materials and expert technical consultation.