Anti-PAK2 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q13177

Core Product Specifications and Parameters

Biological Background: PAK2 Function and Localization

• PAK2 (also known as PAK65, PAKgamma, p58, PAK-2, S6/H4 kinase) is a serine/threonine kinase that acts as a downstream effector of the small GTPases CDC42 and RAC1. It plays a central role in cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation. Related references: PMID:7744004, PMID:12853446, PMID:16617111, PMID:19273597

• Full-length PAK2 promotes cell survival and growth, and phosphorylates JUN to mediate EGF-induced proliferation. It also phosphorylates MAPK4 and MAPK6, activating MAPKAPK5 to regulate F-actin polymerization and cell migration. Related references: PMID:7744004, PMID:21177766, PMID:21317288

• PAK2 phosphorylates numerous substrates including histone H4 to facilitate nucleosome assembly of H3.3-H4, BAD, ribosomal protein S6, and caspase-7 (CASP7), inhibiting CASP7 activity. Related references: PMID:21724829, PMID:21555521, PMID:27889207

• Beyond its kinase activity, PAK2 associates with ARHGEF7 and GIT1 to control spindle orientation during mitosis in a kinase-independent manner. Related references: PMID:19273597, PMID:19923322

• Under apoptotic stimuli, PAK2 is cleaved by caspases to generate a p34 active fragment (PAK-2p34) that translocates to the nucleus and promotes JNK-mediated apoptosis. Caspase-activated PAK2 also phosphorylates MKNK1, reducing cellular translation. Related references: PMID:12853446, PMID:16617111, PMID:9171063, PMID:15234964

• PAK2 localizes to the cytoplasm, nucleus, perinuclear region, and membrane. It is ubiquitously expressed with higher levels in skeletal muscle, ovary, thymus, and spleen.

• Post-translational modifications include acetylation, myristoylation, and regulatory autophosphorylation, all of which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• For Western blotting, detect full-length PAK2 at ~58 kDa; under apoptotic conditions, a 34 kDa caspase-cleaved fragment may appear. Include appropriate positive controls.
• In immunofluorescence/ICC, anticipate cytoplasmic and nuclear staining with possible perinuclear enrichment. Optimize fixation and permeabilization for each cell type.
• For ELISA, titrate against the immunogen peptide (aa 1–100) to confirm specificity; cross-reactivity to mouse and rat suggests broad mammalian utility.
• When using mouse or rat samples, validate reactivity with species-specific positive controls, as cross-reactivity is reported but not quantified.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted IVD raw-material partner, CamelBio streamlines your development from concept to clinic. This PAK2 rabbit monoclonal antibody supports oncology and cell signaling research, and we offer complementary validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to build robust assays. Whether you need rare-target sourcing or customized production, CamelBio bridges your supply requirements with reliable, research-backed solutions.