Anti-PAK1IP1 Polyclonal Antibody for WB, ELISA - Q9NWT1

Core Product Specifications and Parameters

Biological Background: PAK1IP1 Function and Localization

• PAK1IP1 (p21-activated protein kinase-interacting protein 1), also known as PIP1, MAK11, WDR84, or hPIP1, is a WD repeat-containing protein that functions as a negative regulator of PAK1 kinase.
• Inhibits PAK1 activation by binding its N-terminal regulatory domain, thereby preventing CDC42-induced activation and subsequent signaling through MAPK8 and RELA pathways.
• May participate in ribosomal large subunit assembly, contributing to ribosome biogenesis. Related references: PMID:24120868
• Localizes predominantly to the nucleolus within the nucleus, consistent with its role in ribosome production.
• Widely expressed across multiple tissues, including brain, colon, heart, kidney, liver, lung, muscle, peripheral blood leukocytes, placenta, small intestine, spleen, and thymus.
• Classified as a phosphoprotein and signal transduction inhibitor, containing WD repeats that mediate protein-protein interactions.

Experimental Guidance and Technical Tips

• For Western blot analysis, expect a band around 44 kDa corresponding to PAK1IP1; consider optimizing lysate preparation and antibody dilution.
• When using this antibody in ELISA, validate coating conditions and detection system with appropriate controls.
• Confirm cross-reactivity in mouse samples if applicable, though reactivity is indicated; perform pilot experiments to assess suitability.
• The immunogen covers nearly the full-length protein (a.a. 1–392), which may provide broad epitope recognition; still, verify performance in your specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, including validated antibodies like this anti-PAK1IP1 polyclonal antibody for cell signaling and ribosome biogenesis research. Our B2B platform supports diagnostic and research needs with bulk reagents, custom antibody development, and rare target sourcing—streamlining your workflow from concept to clinic.