Anti-PAK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13153

Core Product Specifications and Parameters

Biological Background: PAK1 Function and Localization

• PAK1 is a serine/threonine-protein kinase, also known as alpha-PAK or p65-PAK, that regulates crucial cellular processes including cytoskeletal dynamics, cell adhesion, migration, proliferation, and apoptosis. Related references: PMID:10551809 PMID:11896197 PMID:12876277
• Activated by the Rho GTPases CDC42 and RAC1, PAK1 links these signals to the JNK MAP kinase pathway and phosphorylates MAP2K1 to propagate downstream MAP kinase activation. Related references: PMID:8805275 PMID:9528787
• PAK1 reorganizes the actin cytoskeleton, stress fibers, and focal adhesions, and also regulates microtubule biogenesis by phosphorylating TBCB; it further positively influences microtubule nucleation during interphase. Related references: PMID:9032240 PMID:9395435 PMID:15831477 PMID:27012601
• PAK1 participates in insulin secretion regulation, neuromuscular junction formation via the PAK1-DVL1-MUSK complex, and chemokine receptor trafficking through ACKR2-mediated LIMK1/cofilin phosphorylation. Related references: PMID:22669945 PMID:23633677
• In apoptosis, PAK1 directly phosphorylates BAD to protect cells; its activity is inhibited during apoptosis by binding of CDC2L1 and CDC2L2. Related references: PMID:12624090
• PAK1 phosphorylates RAF1, driving its mitochondrial translocation and BAD phosphorylation, and phosphorylates SNAI1 to enhance its transcriptional repressor activity. Related references: PMID:11733498 PMID:15833848
• In the nucleus, PAK1 responds to DNA damage by phosphorylating MORC2, facilitating chromatin remodeling; in neurons, it stabilizes GABA(A) receptors at synapses and promotes dendritic spine formation. Related references: PMID:23260667
• PAK1 phosphorylates additional targets including FXR1 (stress granule localization), ILK (nuclear export), and is overexpressed in gastric cancer, where it contributes to RUFY3-mediated migration and invasion. Related references: PMID:20417602 PMID:17420447 PMID:25766321

Experimental Guidance and Technical Tips

• The immunogen is designed from amino acids 200–300 of human PAK1; this central region may detect full-length PAK1 and potentially certain isoforms if the epitope is conserved.
• For Western blot (WB), expect a band near 61 kDa; include appropriate positive and negative controls to confirm specificity.
• In immunohistochemistry (IHC-P), antigen retrieval conditions should be optimized; use tissue sections with known PAK1 expression levels as controls.
• For ELISA, the antibody can be employed as capture or detection reagent; pairing with a different clone or antibody may enhance assay sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical services, supporting diagnostic manufacturers from concept to clinic. For PAK1-related research and assay development, we provide validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare target raw materials. Contact us to bridge your procurement needs efficiently.