Anti-PAICS Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P22234

Core Product Specifications and Parameters

Biological Background: PAICS Function and Localization

• Encodes a bifunctional enzyme that catalyzes two sequential ATP-dependent reactions in the de novo purine biosynthetic pathway: phosphoribosylaminoimidazole carboxylase (step 7) and phosphoribosylaminoimidazole succinocarboxamide synthetase (step 8).
• Essential for de novo purine biosynthesis, supplying precursors for DNA and RNA synthesis.
• Alternative splicing generates multiple transcript variants, potentially yielding isoforms with distinct functions.
• Post-translational modifications include acetylation and phosphorylation, which may regulate enzyme activity and subcellular localization.
• Identified by direct protein sequencing and proteomic identification, confirming endogenous expression in human cells.
• Mutations in this gene are associated with disease variants; however, specific disorders are not detailed in this annotation.
• The protein has a molecular weight of approximately 47 kDa (predicted from UniProt and observed in product validation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 176-425 of human PAICS (NP_001072992.1), a region likely spanning the catalytic domain. This design may allow detection of multiple isoforms if the sequence is conserved.
• For Western blot, the expected band is ~47 kDa. Consider using whole-cell lysates from human, mouse, or rat samples as indicated by cross-reactivity.
• In immunofluorescence and IHC, PAICS localization may be cytoplasmic or nuclear depending on cell type and conditions; validate in the relevant sample system.
• ELISA may be employed for quantitative detection; optimize coating antigen and detection parameters.
• Always include appropriate negative and positive controls (e.g., knockout cell lines or siRNA-treated samples) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD antibody raw materials, offering validated antibodies like this anti-PAICS polyclonal for research and diagnostic development in nucleotide metabolism. We supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support rare target sourcing to streamline your assay development.