Anti-PAI-1/Serpin E1 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P05121

Core Product Specifications and Parameters

Biological Background: PAI-1/Serpin E1 Function and Localization

• PAI-1, also known as Serpin E1, is encoded by the SERPINE1 gene and belongs to the serine protease inhibitor (serpin) superfamily.
• It is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU), playing a key role in down-regulating fibrinolysis and controlling blood clot degradation. Related references: PMID:17912461, PMID:8481516, PMID:9207454
• By inhibiting PLAU, PAI-1 regulates cell adhesion and spreading, and it also acts as a regulator of cell migration independently of its protease inhibitor activity. Related references: PMID:9175705, PMID:15001579, PMID:9168821
• The protein is required for keratinocyte migration during cutaneous wound repair and is involved in cellular and replicative senescence. Related references: PMID:18386027, PMID:16862142
• PAI-1 also inhibits TMPRSS7 and participates in odontoblast differentiation and dentin formation during odontogenesis. Related references: PMID:15853774, PMID:25808697, PMID:27046084
• Subcellularly, PAI-1 is secreted and functions in the extracellular environment.
• It is highly expressed in endothelial cells, and is found in plasma, platelets, and hepatoma and fibrosarcoma cells.
• The protein undergoes glycosylation and alternative splicing, contributing to its functional diversity.

Experimental Guidance and Technical Tips

• For western blotting, a band of approximately 45 kDa is expected under reducing conditions. Use endothelial cell lysates or plasma as positive controls.
• In immunofluorescence and immunocytochemistry, consider permeabilization to detect intracellular pools of this secreted protein. Validate in relevant cell types such as HUVECs.
• The immunogen corresponds to amino acids 24–199; verify sequence conservation if using samples from species other than human, mouse, or rat.
• For ELISA, pairing with a suitable capture antibody may enhance sensitivity; titrate the antibody to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material procurement, including this anti-PAI-1 polyclonal antibody, to support research in fibrinolysis and cell migration. We supply validated antibody pairs, bulk reagents, and customized sourcing for rare targets, helping you streamline from concept to clinic. Contact us to explore how our one-stop platform can accelerate your diagnostic development.