Anti-PAF1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q8N7H5

Core Product Specifications and Parameters

Biological Background: PAF1 Function and Localization

• Core component of the PAF1 complex (PAF1C), which regulates transcription elongation by RNA polymerase II, histone modifications, and mRNA 3' end formation.
• PAF1C associates with RNA polymerase II CTD in both non-phosphorylated and phosphorylated states, facilitating coupling between transcription and co-transcriptional processes.
• Promotes histone H2B K120 monoubiquitination (H2BK120ub1) via recruitment of the RNF20/40 ubiquitin ligase complex, and stimulates H3K4me3 methylation; these modifications are critical for active transcription.
• Required for transcription of Hox and Wnt target genes, and plays a role in hematopoiesis and leukemogenesis through interactions with KMT2A/MLL1 fusion oncoproteins.
• Localizes to the nucleus, consistent with its transcriptional regulatory functions.
• Exhibits oncogenic activity; implicated in alternative splicing, phosphorylation, and ubiquitin conjugation pathways.
• During influenza A (H3N2) infection, interacts with viral NS1 protein to modulate host gene transcription.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human PAF1, thus the antibody may detect full-length and certain isoforms. Verify isoform specificity in your experimental system.
• For Western blot, expect a band near 60 kDa under reducing conditions; include positive controls such as HeLa or HEK293 cell lysates.
• IHC-P staining should show nuclear localization; optimize antigen retrieval and antibody dilution using appropriate positive tissue controls.
• In ELISA, the peptide immunogen can serve as a standard. Cross-reactivity with mouse and rat PAF1 allows application across these species; confirm performance in the relevant species’ samples.
• Consider co‑immunoprecipitation or ChIP assays if the antibody functions in native protein complex analysis, but validate those applications independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription and oncology targets like PAF1, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us for customized solutions that streamline your diagnostic development pipeline.