Anti-PAEP Rabbit Polyclonal Antibody for WB, ELISA - P09466

Core Product Specifications and Parameters

Biological Background: Glycodelin Function and Localization

• Glycodelin (PAEP), also known as placental protein 14 (PP14), pregnancy-associated endometrial alpha-2 globulin (PAEG), and zona-binding inhibitory factor-1 (ZIF-1), is a secreted glycoprotein. Four glycoforms—Glycodelin-S, -A, -F, and -C—exhibit distinct glycosylation and biological roles: Glycodelin-A has contraceptive and immunosuppressive activities, Glycodelin-C stimulates sperm-zona binding, Glycodelin-F inhibits sperm-zona binding and the acrosome reaction, and Glycodelin-S maintains sperm in an uncapacitated state. Related references: PMID:7531163 PMID:9918684 PMID:17192260 PMID:12672671 PMID:15883155

• Glycodelin is the major secretory product of the endometrium during the mid-luteal phase and early pregnancy. It exhibits tissue-specific glycoform distribution: Glycodelin-A in amniotic fluid, endometrium/decidua, and maternal serum; Glycodelin-F in follicular fluid, luteinized granulosa cells, and oviduct; Glycodelin-S in seminal plasma and seminal vesicles; and Glycodelin-C in cumulus cells, where it is taken up and glycan-remodeled from Glycodelin-A and -F. Related references: PMID:3667877 PMID:3194393 PMID:12672671 PMID:9239694 PMID:17192260

• The protein is secreted into the extracellular space.

• The predicted molecular weight of the unprocessed precursor is approximately 20.6 kDa (UniProt), and the mature protein migrates at ~21 kDa in SDS-PAGE under reducing conditions.

• Keywords from UniProt include secreted, glycoprotein, disulfide bond, signal peptide, and alternative splicing, indicating post-translational modifications and variant isoforms. A 3D structure has been resolved for this protein.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against the mature region (amino acids 19-180) of human PAEP, likely recognizing multiple epitopes. It should be suitable for detecting both native and denatured Glycodelin in WB and ELISA applications.
• For WB, consider using reducing SDS-PAGE and a positive control such as human endometrial tissue lysate or seminal plasma, as Glycodelin is highly expressed in these tissues. Due to glycosylation, the observed band may appear slightly higher than the predicted 21 kDa. Optimize primary antibody dilution (e.g., 1:500-1:2000) based on sample abundance.
• For ELISA, perform checkerboard titrations to determine the optimal coating concentration and antibody dilution. The antibody may be used for direct or indirect detection formats.
• Cross-reactivity is reported for human only; it is not expected to detect mouse or rat Glycodelin. Validate species specificity in the relevant assay system if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of raw materials for IVD development, including validated antibody pairs, polyclonal antibodies, and bulk ancillary reagents. This Anti-PAEP antibody is suitable for reproductive biology research and immunoassay development, supporting studies on fertilization and immunomodulation. For customized sourcing of rare targets or large-scale production, please contact our technical team.