Anti-PADI1/PAD1 Rabbit Monoclonal Antibody for WB, ELISA - Q9ULC6

Core Product Specifications and Parameters

Biological Background: PADI1/PAD1 Function and Localization

• Protein-arginine deiminase type-1 (PADI1), also known as peptidylarginine deiminase I, is encoded by the PADI1 gene (alternative symbols: PAD1, PDI1).
• Catalyzes the deimination (citrullination) of arginine residues in target proteins, converting positively charged arginine to neutral citrulline.
• Localizes to the cytoplasm, as annotated in UniProt, where it is expected to interact with its substrate proteins.
• Expressed in a tissue-specific manner: highly detected in epidermal keratinocytes and also found in prostate, testis, placenta, spleen, and thymus, suggesting roles in skin biology and other tissues.
• Classified as a hydrolase and a metal-binding protein requiring calcium for activity; its 3D structure has been determined.
• Involved in post-translational modification pathways that can alter protein function and antigenicity.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal 277 amino acids of human PADI1, which may allow detection of both full-length and N-terminal fragments; consider using positive controls expressing full-length protein.
• For Western blot (WB), validate the antibody with a dilution series under reducing conditions and confirm band specificity using lysates from known PADI1-expressing cells or tissues (e.g., keratinocytes).
• In ELISA applications, coat recombinant PADI1 protein or cell lysates and titrate the antibody to determine optimal coating and detection concentrations.
• Given reported cross-reactivity with mouse, this antibody is suitable for translational studies in mouse models; always confirm cross-reactivity experimentally in your system.

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