Anti-PACSIN3 Polyclonal Antibody for WB and ELISA - Q9UKS6

Core Product Specifications and Parameters

Biological Background: PACSIN3 Function and Localization

• PACSIN3 (Protein kinase C and casein kinase substrate in neurons protein 3), also known as SH3 domain-containing protein 6511, is a member of the PACSIN protein family characterized by an N-terminal F-BAR domain and a C-terminal SH3 domain.
• It plays a key role in endocytosis by regulating the internalization of plasma membrane proteins; overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4, increasing their cell surface levels.
• PACSIN3 inhibits TRPV4 calcium channel activity, potentially modulating calcium signaling (by similarity).
• The protein is primarily localized in the cytoplasm and at the cell membrane.
• Widely expressed across tissues, with highest expression in heart and skeletal muscle, intermediate in placenta, liver, and pancreas, and low in brain, lung, and kidney.
• Post-translational modifications include phosphorylation, as indicated by the keyword "Phosphoprotein," which may regulate its activity.
• Contains a coiled-coil domain, suggesting a role in protein-protein interactions or dimerization.
• Associated with disease variants (keyword "Disease variant"), though specific disease implications are not detailed here.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is 48 kDa; primary antibody dilution should be optimized using a control lysate expressing PACSIN3 (e.g., human, mouse, or rat tissue/cell lines).
• For ELISA, consider coating with the immunogen or a peptide derived from the target region; antibody titer should be determined experimentally.
• The immunogen corresponds to a region distinct from the N-terminal F-BAR domain and C-terminal SH3 domain, so this antibody may recognize the middle portion; verify its ability to detect endogenous protein in the relevant sample system.
• Cross-reactivity with mouse and rat suggests sequence conservation in the immunogen region; confirm with your specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw material procurement, covering every stage from concept to clinic. For researchers investigating endocytic regulation and membrane protein trafficking, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support assay development. Our sourcing capabilities extend to rare target raw materials, ensuring comprehensive support for your IVD projects.