Anti-PABPC5 Rabbit Polyclonal Antibody for WB, ELISA - Q96DU9

Core Product Specifications and Parameters

Biological Background: PABPC5 Function and Localization

• PABPC5 encodes polyadenylate-binding protein 5, a member of the PABP family responsible for mRNA poly(A) tail binding and post-transcriptional regulation.
• Binds the poly(A) tail of mRNA and likely participates in cytoplasmic regulatory processes of mRNA metabolism (By similarity).
• May interact with non-poly(A) cytoplasmic RNA sequences, broadening its potential role in mRNA regulation (By similarity).
• Subcellularly located in the cytoplasm and mitochondrial matrix, suggesting dual functions in different cellular compartments.
• Expressed in fetal brain and a range of adult tissues, consistent with a role in RNA metabolism across diverse cell types.
• UniProt keywords include RNA-binding, repeat, and alternative initiation, indicating the presence of RNA recognition motifs and potential isoform diversity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-382, nearly the full-length protein (43 kDa). Thus, the antibody may detect full-length PABPC5 and potentially alternative isoforms arising from alternative initiation.
• For Western blot, lysates from human or mouse brain or other tissues with high PABPC5 expression are appropriate positive controls. Validate specificity using knockdown or knockout samples.
• For ELISA, optimize coating antigen concentration and primary antibody dilution to achieve desired signal-to-noise ratio.
• Consider both reducing and non-reducing conditions to assess if disulfide bonds affect migration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material sourcing, offering validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents for mRNA metabolism research. Our PABPC5 rabbit polyclonal antibody is part of a curated portfolio supporting diagnostic developers and researchers from concept to clinic. Contact us for customized sourcing of rare targets and technical consulting.