Anti-PABPC4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13310

Core Product Specifications and Parameters

Biological Background: PABPC4 Function and Localization

• PABPC4 (Polyadenylate-binding protein 4), also known as activated-platelet protein 1 (APP-1) or inducible poly(A)-binding protein (iPABP), belongs to the poly(A)-binding protein family.
• It binds specifically to the poly(A) tail of mRNA, a key step in mRNA stabilization and translation initiation. Related references: PMID:8524242
• PABPC4 interacts with SMIM26 mRNA and modulates its post-transcriptional regulation, demonstrating its role in specific mRNA metabolism. Related references: PMID:37009826
• It may play a broader role in cytoplasmic regulatory processes of mRNA metabolism, potentially binding non-poly(A) RNA sequences in vivo.
• The protein is primarily localized in the cytoplasm.
• PABPC4 is expressed at low levels in resting normal T cells, but its mRNA levels are rapidly up-regulated upon T-cell activation, indicating a role in immune response regulation.
• Post-translational modifications include methylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation (Ubl), which may regulate its activity and stability.
• The protein has a molecular weight of approximately 70.8 kDa (UniProt) / 71 kDa (observed).

Experimental Guidance and Technical Tips

• For Western blot, a band at approximately 71 kDa is expected; validate using appropriate positive controls (e.g., activated T-cell lysates).
• For IHC-P, consider antigen retrieval conditions; cytoplasmic staining is anticipated.
• For IF/ICC, permeabilization may be necessary to access the cytoplasmic PABPC4.
• Due to its polyclonal nature, this antibody can be suitable for ELISA as a detection antibody.
• Cross-reactivity with mouse and rat suggests sequence conservation; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. With expertise in antibody development and supply, we offer validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. For mRNA biology and immune regulation research, CamelBio is your trusted partner for high-quality PABPC4 antibodies and related raw materials.