Anti-p75NTR/NGFR Rabbit PolymAb® for WB, IHC-P, ELISA - P08138 / Q9Z0W1

Core Product Specifications and Parameters

Biological Background: p75NTR/NGFR Function and Localization

• Encodes tumor necrosis factor receptor superfamily member 16 (TNFRSF16), also known as low-affinity nerve growth factor receptor (LNGFR) or p75NTR.
• Serves as a low-affinity receptor for mature neurotrophins (NGF, BDNF, NTF3, NTF4) and forms a high-affinity heterodimeric receptor with SORCS2 for precursor neurotrophins. Related references: PMID:24908487
• Plays critical roles in neuronal fate determination, capable of mediating both cell survival and apoptosis, and inactivates RHOA. Related references: PMID:26646181
• Regulates insulin-dependent glucose uptake by modulating GLUT4 translocation through RAB31 in adipocytes and skeletal muscle (By similarity).
• Required for circadian rhythm maintenance by regulating clock gene expression (BMAL1, PER1, PER2, NR1D1) in the suprachiasmatic nucleus and liver, and influences metabolic gene regulation. Related references: PMID:23785138
• Together with BFAR, negatively regulates NF-kappa-B and JNK-related pro-inflammatory signaling pathways. Related references: PMID:22566094
• Subcellularly localizes to the cell membrane, cytoplasm, perikaryon, growth cones, and dendritic spines, reflecting its diverse signaling functions.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; alternative splicing yields isoforms such as soluble p75ICD.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–400 within the intracellular domain, suggesting recognition of full-length and possibly truncated p75ICD isoforms; validate specificity using isoform-specific controls.
• For western blot, anticipate a band near 45 kDa; confirm identity with positive controls (e.g., neuronal cell lines) and negative controls (e.g., p75NTR knockout lysates if available).
• In IHC-P, optimal antigen retrieval and detection may require titrating antibody concentration; evaluate using known p75NTR-expressing tissues (e.g., basal forebrain, Schwann cells).
• For ELISA, test binding to recombinant p75NTR extracellular domain or peptide to assess affinity; include cross-reactivity checks with related TNFRSF members.
• Cross-reacts with human, mouse, and rat; verify species-specific reactivity in your experimental system and consider blocking conditions to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions by providing validated antibody pairs, optimized monoclonal and polyclonal reagents, and ancillary raw materials for IVD development. For neurotrophin receptors and cell signaling targets like p75NTR/NGFR, we offer scalable supply solutions—from discovery-grade antibodies to bulk-ready conjugates—enabling your transition from concept to clinical application. Contact our team to explore tailored sourcing for rare-target raw materials and comprehensive technical collaboration.