Anti-p75NTR/NGFR (CD271) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P08138

Core Product Specifications and Parameters

Biological Background: p75NTR/NGFR Function and Localization

• Encoded by the NGFR gene, p75NTR/NGFR (CD271/TNFRSF16) is a single-pass type I membrane glycoprotein and member of the tumor necrosis factor receptor superfamily. It is also known as low-affinity nerve growth factor receptor (LNGFR) or Gp80-LNGFR.

• Acts as a low-affinity receptor for mature neurotrophins NGF, BDNF, NTF3, and NTF4, and forms a high-affinity heterodimeric receptor with SORCS2 for precursor forms of NGF, BDNF, and NTF3. Related references: PMID:24908487

• Regulates diverse neuronal functions including differentiation, survival, and programmed cell death of specific neuronal populations during development. Signaling can promote either survival or apoptosis depending on cellular context.

• Plays a role in the inactivation of RHOA, contributing to cytoskeletal dynamics. Related references: PMID:26646181

• Involved in insulin-responsive GLUT4 translocation to the cell surface in adipocytes and skeletal muscle cells, thereby regulating glucose uptake.

• Required for circadian oscillation of clock genes (BMAL1, PER1, PER2, NR1D1) in the suprachiasmatic nucleus and liver, and modulates hepatic glucose and lipid metabolism genes. Related references: PMID:23785138

• Together with BFAR, negatively regulates NF-kappa-B and JNK signaling pathways, influencing inflammatory and stress responses. Related references: PMID:22566094

• Subcellularly, p75NTR/NGFR localizes to the cell membrane, cytoplasm, perikaryon, growth cones, and dendritic spines. It undergoes N-glycosylation and phosphorylation, and exists in multiple alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 300–400 within the intracellular domain; the antibody may perform optimally in applications where the epitope is accessible (e.g., denaturing WB).
• For western blot, consider using positive control lysates from human, mouse, or rat neuronal tissues or cell lines (e.g., SH-SY5Y, PC12) expressing p75NTR/NGFR.
• In IHC-P, antigen retrieval conditions should be optimized; the antibody is expected to detect both full-length and truncated isoforms.
• For ELISA, peptide-based assays or recombinant protein capture may be considered; validate with appropriate standards.
• Cross-reactivity with mouse and rat is reported, but potential species-specific differences in epitope recognition should be verified experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research institutes, and core labs by providing a streamlined supply of validated IVD raw materials. This anti-p75NTR/NGFR monoclonal antibody exemplifies our expertise in delivering high-quality rabbit monoclonal antibodies for neurotrophin signaling studies. We offer one-stop access to optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, helping you accelerate your assay development from concept to clinic.