Anti-p70 S6 Kinase 2 Polyclonal Antibody for WB, ELISA - Q9UBS0

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 2 Function and Localization

• Ribosomal protein S6 kinase beta-2 (RPS6KB2), also known as p70 S6 kinase beta (p70 S6K-beta), p70-beta, S6K2, and STK14B, is a serine/threonine-protein kinase belonging to the ribosomal S6 kinase family.
• It specifically phosphorylates ribosomal protein S6, a component of the 40S ribosomal subunit, thereby regulating protein synthesis and cell growth (PubMed:29750193). Related references: PMID:29750193
• Acts downstream of mTOR signaling in response to growth factors and nutrients, promoting cell proliferation, cell growth, and cell cycle progression through the MEAK7-dependent alternative pathway.
• Localizes to both the cytoplasm and nucleus, where it may exert distinct functions in translation regulation and nuclear signaling.
• Possesses ATP-binding, nucleotide-binding, and transferase activities characteristic of kinases; undergoes alternative splicing and phosphorylation, as identified by proteomics.
• As a phosphoprotein subject to alternative splicing, it may be regulated by post-translational modifications and exhibit isoform diversity, as highlighted by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-170), suggesting that the antibody recognizes epitopes within this domain. Consider using target-specific peptides or recombinant fragments for blocking experiments to confirm specificity.
• For Western blot, validate in lysates from human, mouse, and rat cell lines (e.g., HeLa, HEK293, NIH/3T3) as the antibody shows cross-reactivity. The predicted molecular weight is ~53 kDa, but phosphorylation may cause band shifts.
• In ELISA, optimize coating antigen concentration and detection conditions; use appropriate negative controls to minimize non-specific binding.
• As a polyclonal antibody, it may offer robust signal but lot-to-lot variability should be evaluated. Include appropriate positive and negative controls in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For researchers and diagnostic developers focusing on cell signaling and cancer biology, we provide reliable access to p70 S6 Kinase 2 antibodies and related reagents to accelerate your assay development and manufacturing workflows.