Anti-p70 S6 Kinase 1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P23443

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 1 Function and Localization

• p70 S6 Kinase 1 (encoded by RPS6KB1, also known as S6K, S6K1, STK14A, S6K-beta-1) is a serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth, and cell cycle progression. Related references: PMID:11500364 PMID:12801526 PMID:14673156
• It regulates protein synthesis by phosphorylating translation factors EIF4B, RPS6, and EEF2K, and it controls translation initiation by targeting PDCD4 for degradation, thereby facilitating ribosome recruitment and pioneer rounds of translation. Related references: PMID:11500364 PMID:16286006 PMID:17053147 PMID:15341740
• Phosphorylates mitochondrial URI1, leading to dissociation of URI1-PPP1CC complex; the free PPP1CC dephosphorylates RPS6KB1 at Thr-412 in a negative feedback loop for anti-apoptotic signaling. Related references: PMID:17936702
• It participates in feedback regulation of mTORC1 and mTORC2 by phosphorylating components like RICTOR, MTOR, DEPTOR, and MAPKAP1/SIN1, thereby modulating AKT signaling. Related references: PMID:15899889 PMID:19720745 PMID:19935711 PMID:22017876
• Under nutrient depletion, the inactive form associates with the EIF3 translation initiation complex; upon mitogenic stimulation and mTORC1 phosphorylation, it dissociates and becomes activated. Related references: PMID:16286006
• Subcellular localization includes cytoplasm, nucleus, mitochondrial outer membrane, and synapse/synaptosome, reflecting its diverse functions in translation, metabolism, and neuronal signaling.
• Alternative splicing and alternative initiation produce multiple isoforms; the protein is subject to acetylation and extensive phosphorylation.
• It is widely expressed across tissues, consistent with its central role in growth control.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 421–520 of human p70 S6 Kinase 1) lies within the kinase catalytic domain; consider that immunoreactivity may depend on the accessibility of this epitope in various assay formats.
• For Western blot, a band around 59 kDa is expected; multiple isoforms may be detected due to alternative splicing and post-translational modifications.
• Cross-reactivity with mouse and rat has been observed; validate in your specific model system to confirm reactivity and specificity.
• This polyclonal antibody is suitable for detecting total p70 S6 Kinase 1; for phosphorylated forms, a phospho-specific antibody may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This p70 S6 Kinase 1 antibody supports research into mTOR signaling, cell proliferation, and cancer biology. For developers requiring validated antibody pairs, bulk ancillary reagents, or rare target sourcing, CamelBio streamlines your procurement and assay development needs.