Anti-p70 S6 Kinase 1 Monoclonal Antibody for WB, IF/ICC, ELISA - P23443

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 1 Function and Localization

• Ribosomal protein S6 kinase beta-1 (RPS6KB1), also known as p70 S6 kinase 1 (p70S6K1), S6K, and STK14A, is a serine/threonine-protein kinase encoded by the RPS6KB1 gene in human (GeneID: 6198).
• Functions as a critical downstream effector of mTOR signaling, promoting cell proliferation, growth, and cell cycle progression in response to growth factors and nutrients. Related references: PMID:11500364 PMID:12801526 PMID:14673156
• Regulates protein synthesis through phosphorylation of EIF4B, RPS6, and EEF2K; also enhances translation initiation by targeting PDCD4 for degradation and phosphorylating POLDIP3/SKAR. Related references: PMID:11500364 PMID:12801526 PMID:14673156 PMID:17053147 PMID:15341740
• Primarily localizes to the cytoplasm and nucleus, but also detected at mitochondria outer membrane, synapse, and synaptosome.
• Widely expressed across tissues.
• Phosphorylated at multiple sites and acetylated; involved in feedback inhibition of mTORC2 by phosphorylating SIN1, mTOR, RICTOR, and DEPTOR; also suppresses pro-apoptotic BAD and regulates insulin signaling via IRS1 phosphorylation. Related references: PMID:15899889 PMID:19720745 PMID:19935711 PMID:22017876 PMID:17936702 PMID:18952604

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human p70 S6 Kinase 1 (NP_003152.1). This N-terminal targeting may be useful for detecting both full-length and potential N-terminally truncated isoforms.
• For Western blot (WB), a band at approximately 59 kDa is expected in human, mouse, and rat samples; cell lines or tissues with active mTOR signaling (e.g., stimulated HeLa, HEK293, or liver tissue) may serve as positive controls.
• In immunofluorescence (IF/ICC), punctate cytoplasmic and nuclear staining may be observed, with possible mitochondrial or synaptic localization under specific conditions; fixation and permeabilization should be optimized.
• For ELISA, the antibody can be considered as a capture or detection reagent in sandwich assays, but pairing validation and titer determination are recommended in the relevant sample matrix.
• Antibody specificity should be confirmed using knockout/knockdown models or phosphatase treatment to verify phospho-dependence, as the target is heavily phosphorylated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-p70 S6 Kinase 1 monoclonal antibody supports research in cell growth, cancer, and mTOR signaling pathways. For rare or custom target sourcing, our team can assist with tailored procurement and technical consultation.