Anti-p63 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9H3D4

Core Product Specifications and Parameters

Biological Background: p63 Function and Localization

• p63, also known as Tumor protein 63, is a member of the p53 family of transcription factors, with multiple isoforms generated by alternative promoter usage and alternative splicing, including TA and DeltaN isoforms that differ in their N-terminal transactivation domains.
• Functions as a sequence-specific DNA-binding transcriptional activator or repressor, regulating genes involved in epithelial morphogenesis, apoptosis, and development. Isoform 2 activates RIPK4 transcription.
• Plays a critical role in limb formation from the apical ectodermal ridge and is required for proper epithelial stratification and maintenance of epithelial stem cell compartments.
• Involved in Notch signaling by probably inducing JAG1 and JAG2, and may be required in conjunction with TP73/p73 for initiation of TP53-dependent apoptosis in response to genotoxic stress.
• Binds to an upstream regulatory region of the DSG1 gene and promotes its transcription, contributing to epithelial differentiation. Related references: PMID:24594668
• Localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Widely expressed in tissues including heart, kidney, placenta, prostate, skeletal muscle, testis, and thymus, with tissue-specific isoform expression. DeltaN isoforms are highly expressed in progenitor cell layers of skin, breast, eye, and prostate. Isoform 10 is predominantly found in skin squamous cell carcinomas.
• Keywords: DNA-binding, metal-binding (zinc), transcription regulation, developmental protein, Notch signaling, apoptosis, disease variant (associated with ectodermal dysplasia), and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-203 of human p63) covers the N-terminal region, which includes the transactivation domain present in TA isoforms; thus, the antibody may detect both TA and DeltaN isoforms depending on epitope location. Consider validating isoform specificity in your system.
• For Western blotting, bands in the 45-77 kDa range are expected, corresponding to various p63 isoforms. Use appropriate positive controls (e.g., human or mouse cell lines known to express p63).
• In IHC-P, nuclear staining is anticipated; optimization of antigen retrieval and antibody dilution may be necessary for your tissue type.
• ELISA can be used for quantitative detection; ensure the assay format is compatible with the antibody isotype.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research institutions with reliable access to antibodies like this anti-p63 monoclonal antibody. Whether you need validated antibody pairs, bulk ancillary reagents, or rare target raw materials for p53 family transcription factors and developmental biology research, CamelBio streamlines procurement from concept to clinic.