Anti-p63 Rabbit Monoclonal Antibody for IHC-P, ELISA - Q9H3D4

Core Product Specifications and Parameters

Biological Background: p63 Function and Localization

• p63 (TP63) is a member of the p53 transcription factor family, with multiple isoforms generated by alternative promoter usage and alternative splicing, including transactivating (TA) and N-terminally truncated (DeltaN) variants.
• Functions as a sequence-specific DNA-binding transcriptional activator or repressor; isoform-specific activity is determined by the presence of transactivation and auto-regulatory domains.
• Plays a critical role in epithelial morphogenesis and stratification; the ratio of DeltaN and TA isoforms regulates maintenance of epithelial stem cell compartments and initiation of stratification from embryonic ectoderm.
• Involved in Notch signaling, likely by inducing JAG1 and JAG2, and participates in p53-dependent apoptosis when cooperating with TP73/p73 in response to genotoxic stress or oncogene activation.
• Known target genes include CDKN1A (p21) and DSG1; it promotes DSG1 transcription by binding to its upstream regulatory region. Related references: PMID:24594668
• Localizes to the nucleus; widely expressed in tissues such as heart, kidney, placenta, prostate, skeletal muscle, testis, and thymus, with high DeltaN-type expression in progenitor cell layers of skin, breast, eye, and prostate. Disease associations include ectodermal dysplasia and premature ovarian failure.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide within amino acids 581–680 of human p63 (NP_003713.3); verify target isoform expression in your samples as this region may not be present in all variants.
• For IHC-P applications, consider optimizing antigen retrieval and antibody dilution; p63 nuclear localization should be assessed with appropriate nuclear counterstain.
• For ELISA, standard protocols using peptide antigen or recombinant protein can be employed; validate assay conditions for your specific requirements.
• Cross-reactivity with mouse and rat is reported; confirm performance in these species under your experimental conditions before large-scale studies.
• This product is validated for IHC-P and ELISA; applications such as Western blotting or immunoprecipitation have not been characterized and would require further testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio from validated monoclonal antibodies and optimized antibody pairs to bulk ancillary reagents. For researchers exploring the roles of p63 in epithelial morphogenesis, stem cell biology, or oncology, we provide reliable access to high-quality reagents. Contact our team for custom sourcing solutions or technical consultation to accelerate your project from concept to clinic.