Anti-p60 CAF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13112

Core Product Specifications and Parameters

Biological Background: p60 CAF1 Function and Localization

• Encoded by the CHAF1B gene, also known as CAF1A, CAF1P60, MPHOSPH7; designated Chromatin assembly factor 1 subunit B, the p60 subunit of CAF-1.
• Core component of the CAF-1 histone chaperone complex, which facilitates deposition of newly synthesized histones H3 and H4 onto replicating DNA during replication and repair.
• Essential for the first step of nucleosome assembly, enabling subsequent binding of histones H2A/H2B to complete the histone octamer.
• Localizes to both the nucleus and cytoplasm; nuclear accumulation is consistent with its chromatin assembly function.
• Subject to post-translational modifications including phosphorylation (originally identified as an M-phase phosphoprotein) and acetylation, suggesting cell cycle regulation.
• Contains WD repeat domains, characteristic of proteins that mediate protein-protein interactions and complex assembly.
• Implicated in DNA damage response and repair pathways, ensuring chromatin structure restoration after genotoxic stress.
• Experimentally determined molecular weight of approximately 61 kDa (UniProt: 61493 Da).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 270–559 of p60 CAF1, covering a central region that may recognize both full-length and potential isoforms.
• For Western blot, expect a band around 61 kDa; consider using nuclear/cytoplasmic fractionation to enrich the target and appropriate loading controls.
• IHC-P may benefit from antigen retrieval optimization; validate staining in known proliferative tissues and include isotype controls.
• ELISA suitability suggests detection of native or recombinant p60 CAF1; confirm linearity and specificity with recombinant protein standards.
• Cross-reactivity with mouse and rat implies sequence conservation, but verification in your specific experimental model is recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio sources high-affinity antibodies like this anti-p60 CAF1 polyclonal antibody to empower chromatin biology and DNA replication research. We offer stable supply of validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials—helping diagnostic developers and laboratories advance from concept to clinic efficiently. Whether you need routine reagents or rare-target sourcing, CamelBio bridges procurement gaps with a reliable one-stop approach.