Anti-p60 CAF1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q13112

Core Product Specifications and Parameters

Biological Background: CHAF1B Function and Localization

• Encoded by the CHAF1B gene, the protein is also known as Chromatin assembly factor 1 subunit B, CAF-I 60 kDa subunit (CAF-I p60), and M-phase phosphoprotein 7.
• Functions as a core component of the Chromatin Assembly Factor 1 (CAF-1) complex, a histone chaperone that mediates the first step of nucleosome assembly by depositing newly synthesized histones H3 and H4 onto replicating DNA.
• Essential for DNA replication-coupled chromatin assembly and DNA repair, ensuring proper packaging of newly synthesized DNA into chromatin.
• The CAF-1 complex is conserved across eukaryotes and plays a critical role in maintaining epigenetic information during cell division.
• Subcellular localization is primarily nuclear, consistent with its role in DNA replication and repair; however, it can also shuttle to the cytoplasm.
• Contains WD repeats that mediate protein-protein interactions within the CAF-1 complex and with other chromatin factors.
• Post-translational modifications include acetylation and phosphorylation (notably during M phase), which may regulate its activity and interactions.
• Acts as a phosphoprotein, with phosphorylation potentially modulating its function during the cell cycle.

Experimental Guidance and Technical Tips

• The antibody was raised against amino acids 459-559 of human p60 CAF1. Confirm that this epitope is present in your target species and isoform.
• For Western blot, use appropriate loading controls and validate the antibody in your specific sample lysate; the predicted molecular weight is approximately 61 kDa.
• For immunohistochemistry (paraffin-embedded sections), antigen retrieval may be required; optimization of retrieval buffer and conditions is recommended.
• In ELISA, coat the antigen under appropriate conditions and consider using a blocking buffer to reduce nonspecific binding.
• Cross-reactivity has been validated for human; if using in other species, sequence homology should be checked and validation performed.
• Always include positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a wide range of validated antibodies, bulk ancillary reagents, and custom sourcing solutions. This anti-p60 CAF1 antibody supports research into chromatin biology, DNA replication, and repair mechanisms, critical areas for diagnostic assay development. Our supply network ensures reliable access to high-quality monoclonal antibodies and related raw materials to accelerate your diagnostic workflow from concept to clinic.