Anti-P4HB Rabbit PolymAb® for WB, IP, IF/ICC, IHC-P, ELISA - P07237

Core Product Specifications and Parameters

Biological Background: P4HB (Protein Disulfide-Isomerase) Function and Localization

• P4HB encodes protein disulfide-isomerase (PDI), also known as PDIA1, ERBA2L, and other aliases. It is a multifunctional enzyme belonging to the protein disulfide isomerase family.
• PDI catalyzes the formation, breakage, and rearrangement of disulfide bonds. At the cell surface, it functions as a reductase that cleaves disulfide bonds of attached proteins, potentially modifying exofacial proteins. Intracellularly, it ensures correct disulfide bond formation in nascent proteins.
• At high concentrations and following phosphorylation by FAM20C, PDI acts as a chaperone that inhibits aggregation of misfolded proteins. Conversely, at low concentrations, it facilitates aggregation (anti-chaperone activity). Related references: PMID:32149426
• PDI serves as a structural subunit of prolyl 4-hydroxylase and the microsomal triacylglycerol transfer protein MTTP, contributing to collagen synthesis and lipid metabolism.
• It functions as a receptor for LGALS9 on Th2 T helper cells, an interaction that retains P4HB at the cell surface, enhances disulfide reductase activity, alters the plasma membrane redox state, and promotes cell migration. Related references: PMID:21670307
• Subcellular localization includes the endoplasmic reticulum lumen, melanosomes, and the cell membrane, reflecting its diverse roles in protein folding, secretion, and cell surface redox regulation.
• Post-translational modifications include acetylation and phosphorylation; it contains a redox-active center with disulfide bonds, and shares structural features such as thioredoxin-like domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 329–508 (C-terminal region) of human P4HB, which may confer specificity towards PDI and reduce cross-reactivity with other disulfide isomerase family members.
• For Western blot (WB), expect a band at approximately 57 kDa. Validate the antibody in your specific sample system, as post-translational modifications may cause minor shifts.
• In immunoprecipitation (IP) and immunofluorescence (IF/ICC), native protein conformation may be recognized; optimize lysis and fixation conditions to preserve epitopes.
• For immunohistochemistry (IHC-P), consider appropriate antigen retrieval methods, as the epitope is located in a structured domain.
• The antibody is reported to cross-react with mouse and rat P4HB, but validation in the desired species/tissue is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. This anti-P4HB antibody supports research into protein folding, ER stress, and disulfide isomerase-related pathways. Whether you need large-scale production or customized technical support, CamelBio bridges the gap from concept to clinic.