Anti-P4HB Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P07237

Core Product Specifications and Parameters

Biological Background: P4HB Function and Localization

• P4HB encodes Protein disulfide-isomerase (PDI), a multifunctional endoplasmic reticulum (ER) chaperone and oxidoreductase that catalyzes disulfide bond formation, breakage, and rearrangement in nascent proteins.
• It also functions as a cell-surface reductase, cleaving exofacial protein disulfide bonds and modulating plasma membrane redox state, particularly in Th2 cells via interaction with LGALS9. Related references: PMID:21670307
• P4HB serves as a structural subunit of several multimeric enzymes, including prolyl 4-hydroxylase (involved in collagen synthesis) and microsomal triacylglycerol transfer protein (MTTP), highlighting its role in diverse biosynthetic pathways.
• At high concentrations and upon phosphorylation by FAM20C, P4HB acts as a chaperone to inhibit misfolded protein aggregation, while at low concentrations it facilitates aggregation, demonstrating concentration-dependent anti-chaperone activity. Related references: PMID:32149426
• The protein contains a redox-active CXXC motif and two thioredoxin-like domains, consistent with its isomerase and chaperone functions. It is localized primarily in the ER lumen but also detected at the plasma membrane and in melanosomes.
• Post-translational modifications include acetylation and phosphorylation, which regulate its chaperone activity. Mutations in P4HB are associated with diseases such as osteogenesis imperfecta and craniosynostosis, underlining its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 329–508) of human P4HB; the resulting polyclonal antibody may recognize full-length endogenous protein across multiple species.
• For Western blotting, a major band at approximately 57 kDa is expected in human, mouse, and rat samples; consider using reduced denaturing conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate the antibody with appropriate positive and negative control tissues/cells, as P4HB is abundantly expressed in the ER.
• The antibody is also suitable for ELISA; in-house titration and optimization are recommended for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw material sourcing partner, offering validated antibodies, antibody pairs, and bulk ancillary reagents to support diagnostic development from concept to clinic. This rabbit polyclonal anti-P4HB antibody targets a key ER chaperone and enzyme subunit widely studied in protein folding disorders, cancer, and fibrosis. Contact us for technical consulting and custom sourcing solutions for this target or any rare marker in your pipeline.