Anti-P4HB Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07237

Core Product Specifications and Parameters

Biological Background: P4HB Function and Localization

• Protein disulfide-isomerase (P4HB) is a multifunctional enzyme and chaperone of the endoplasmic reticulum, also known as PDI, PDIA1, and prolyl 4-hydroxylase subunit beta.
• Catalyzes the formation, breakage, and rearrangement of disulfide bonds in nascent proteins, ensuring proper protein folding in the ER lumen.
• At the cell surface, acts as a reductase that cleaves disulfide bonds of exofacial proteins, modulating cell surface protein structure and function. Related references: PMID:21670307
• Upon phosphorylation by FAM20C, high concentrations of P4HB function as a chaperone inhibiting aggregation of misfolded proteins, while low concentrations promote aggregation (anti-chaperone activity). Related references: PMID:32149426
• Serves as a structural subunit of the prolyl 4-hydroxylase complex and the microsomal triacylglycerol transfer protein MTTP.
• Subcellular localization includes endoplasmic reticulum lumen, melanosomes, and the cell membrane; it is also secreted to the cell surface where it interacts with LGALS9.
• Keywords and disease associations: mutations in P4HB are linked to craniosynostosis and osteogenesis imperfecta; post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 409-508) of human P4HB; epitope accessibility may be influenced by protein folding or interactions.
• For Western blotting (WB), a band around 57 kDa is expected. Use appropriate loading controls and validate antibody specificity using knockdown or knockout samples.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) may enhance signal. Optimize primary antibody concentration and incubation conditions.
• For ELISA, consider pairing this monoclonal antibody with a polyclonal capture or detection antibody for sandwich assays; validate in the relevant sample matrix.
• Cross-reactivity with mouse and rat is reported; however, verify sequence homology of the immunogen region across species to confirm binding if using non-human samples.
• Store antibody as recommended (ice bag shipping indicates cold chain requirement); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. The P4HB Rabbit Monoclonal Antibody is part of our extensive catalog of validated antibodies for protein folding and redox biology research. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials—supporting your assay development and large-scale manufacturing needs.