Anti-P4HA3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7Z4N8

Core Product Specifications and Parameters

Biological Background: P4HA3 Function and Localization

• P4HA3 (prolyl 4-hydroxylase subunit alpha-3) is an enzyme critical for collagen biosynthesis, catalyzing the formation of 4-hydroxyproline in -Xaa-Pro-Gly- motifs.
• This post-translational modification is essential for the thermal stability of the collagen triple helix and proper extracellular matrix assembly.
• Multiple isoforms are generated by alternative splicing, and the protein features a signal peptide, tetratricopeptide (TPR) repeats, and a coiled-coil domain.
• Localized primarily to the endoplasmic reticulum lumen, where collagen processing occurs.
• Highly expressed in placenta, liver, and fetal skin; also detected in atherosclerotic lesion fibrous caps and at lower levels in other tissues.
• Belongs to the P4HA family of 2-oxoglutarate- and iron-dependent dioxygenases; requires vitamin C for activity.
• Post-translational modifications include glycosylation, and the protein is known to bind iron as a cofactor.

Experimental Guidance and Technical Tips

• The immunogen (aa 20–110) targets the N-terminal region; perform sequence alignment to predict cross-reactivity in additional species.
• For Western blot, the predicted molecular weight is 61 kDa; validate using positive control lysates from placenta or liver.
• For IF/ICC, optimize permeabilization conditions to detect the ER lumen-resident protein.
• ELISA may require titration of both coating antigen and antibody to achieve optimal signal.

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