Anti-P4HA2 Rabbit Monoclonal Antibody for WB, ELISA - O15460

Core Product Specifications and Parameters

Biological Background: P4HA2 Function and Localization

• P4HA2 (Prolyl 4-hydroxylase subunit alpha-2) is a key catalytic subunit of the prolyl 4-hydroxylase enzyme complex, which catalyzes the post-translational formation of 4-hydroxyproline in collagens and other proteins containing -Xaa-Pro-Gly- sequences.
• This hydroxylation is critical for the stability of the collagen triple helix and proper extracellular matrix assembly.
• The enzyme localizes to the endoplasmic reticulum lumen, where collagen processing and modification occur.
• P4HA2 is expressed in several tissues, including heart, placenta, lung, and pancreas, suggesting roles in organ-specific matrix remodeling.
• The protein requires iron and vitamin C as cofactors for its dioxygenase activity and is subject to glycosylation.
• Alternative splicing generates multiple isoforms, potentially with distinct biological functions.
• Mutations or dysregulation of P4HA2 are associated with fibrosis and other connective tissue disorders; it is listed as a disease variant in UniProt.
• Keywords: Glycoprotein, Iron, Metal-binding, Oxidoreductase, TPR repeat, Vitamin C, Alternative splicing, Disease variant.

Experimental Guidance and Technical Tips

• The immunogen used (amino acids 157-333) may recognize multiple P4HA2 isoforms; consider verifying reactivity with the specific isoform of interest.
• For Western blot, the predicted molecular weight of P4HA2 is approximately 61 kDa. However, post-translational modifications may cause slight shifts; validate with appropriate controls and a positive lysate from a tissue expressing P4HA2 (e.g., placenta or heart).
• This antibody is validated for ELISA; when developing sandwich immunoassays, pairing with a different anti-P4HA2 antibody may improve sensitivity and specificity. Titrate both capture and detection antibodies in the relevant matrix.
• Cross-reactivity has been confirmed for human P4HA2; other species have not been tested. If targeting non-human samples, evaluate sequence homology in the immunogen region.
• For immunohistochemistry or other applications not listed, test the antibody first under appropriate conditions, as performance may vary with fixation and antigen retrieval methods.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to deliver a comprehensive range of IVD raw materials, technical services, and consulting—from early-stage development to commercialization. This anti-P4HA2 rabbit monoclonal antibody supports studies on collagen biology, fibrosis, and oxygen sensing, with potential applications in biomarker discovery and assay development. Beyond individual antibodies, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and access to rare targets, ensuring your supply chain is reliable and scalable.