Anti-P4HA1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13674

Core Product Specifications and Parameters

Biological Background: P4HA1 Function and Localization

• Prolyl 4-hydroxylase subunit alpha-1 (P4HA1), also known as procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, is a catalytic subunit of the collagen prolyl 4-hydroxylase enzyme complex.
• It catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins, essential for collagen triple-helix stability.
• P4HA1 localizes to the endoplasmic reticulum lumen, where it modifies nascent procollagen chains during biosynthesis.
• The protein is expressed in multiple tissues including the heart, liver, skeletal muscle, kidney, placenta, lung, and pancreas.
• As a dioxygenase, P4HA1 requires iron and vitamin C as cofactors and is a member of the 2-oxoglutarate-dependent oxygenase family.
• Post-translational modifications include glycosylation, and the gene undergoes alternative splicing, potentially generating multiple isoforms.
• Keywords: 3D-structure, Alternative splicing, Dioxygenase, Endoplasmic reticulum, Glycoprotein, Iron, Metal-binding, Oxidoreductase, Proteomics identification, Reference proteome, Signal, TPR repeat, Vitamin C.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide encompassing amino acids 235-534 of human P4HA1. Antibody specificity may be confirmed by peptide competition ELISA or Western blot using recombinant P4HA1 protein.
• For Western blot, the predicted molecular weight is ~61 kDa; validate with positive control lysates from human, mouse, or rat cell lines expressing P4HA1.
• In immunofluorescence/ICC, P4HA1 is expected to localize to the endoplasmic reticulum; use appropriate co-staining (e.g., ER markers) to confirm.
• For ELISA, consider a direct or sandwich format and titrate the antibody for optimal signal with the immunizing peptide or full-length protein.
• Antibody performance may vary by application; pilot studies with known positive/negative controls are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, connecting diagnostic developers and researchers with high-quality critical components. For anti-P4HA1 polyclonal antibodies and other collagen-modifying enzyme targets, CamelBio streamlines procurement from concept to clinic. Our platform also supports customized sourcing for rare or specialized biomolecules to accelerate your assay development.