Anti-p47phox/NCF1 Rabbit Monoclonal Antibody for WB, ELISA - P14598

Core Product Specifications and Parameters

Biological Background: p47phox/NCF1 Function and Localization

• p47phox (Neutrophil cytosol factor 1) is encoded by the NCF1 gene (also known as NOXO2, SH3PXD1A) and is a 47 kDa cytosolic protein; alternative splicing has been reported but not fully characterized.
• As a subunit of the phagocyte NADPH oxidase complex, p47phox enables electron transfer from cytosolic NADPH to O2 to produce superoxide anion (O2(-)). Related references: PMID:2547247, PMID:2550933, PMID:38355798
• Activation involves phosphorylation-dependent binding of p47phox to the C-terminus of CYBA/p22-phox, initiating assembly of cytosolic subunits with the core membrane complex. Related references: PMID:12732142, PMID:19801500
• p47phox is primarily located in the cytoplasm and cytosol, and translocates to the membrane upon activation.
• Detected at the protein level in peripheral blood monocytes and neutrophils.
• Features include SH3 domains, a PX domain for lipid binding, and phosphorylation sites, consistent with its regulatory role.
• Defects in NCF1 are associated with autosomal recessive chronic granulomatous disease (CGD), characterized by impaired phagocytic killing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 291–390 of human p47phox; if cross-reactivity to other species is observed, consider validating detection in those systems.
• For Western blot, a band at approximately 45 kDa is expected; include appropriate molecular weight markers and loading controls.
• Validate the antibody in physiologically relevant sample types, such as human peripheral blood monocytes or neutrophils.
• For ELISA applications, optimize antibody dilution and consider appropriate blocking and washing conditions for robust signal detection.
• When detecting phosphorylated forms, note that activation-dependent phosphorylation may affect band shift or signal intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-p47phox/NCF1 antibody is tailored for research on NADPH oxidase function and CGD-related pathways. We also facilitate procurement of rare-target raw materials, providing a seamless link from discovery to diagnostic development.