Anti-p47phox/NCF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P14598

Core Product Specifications and Parameters

Biological Background: p47phox/NCF1 Function and Localization

• Also known as Neutrophil cytosol factor 1, NCF-47K, Nox organizer 2 (NOXO2), and p47-phox. It is a subunit of the phagocyte NADPH oxidase complex.
• As part of the NADPH oxidase complex, it mediates electron transfer from NADPH to O2 to produce superoxide anion (PubMed:2547247, PubMed:2550933, PubMed:38355798). This superoxide generation is critical for the microbicidal activity of phagocytes. Related references: PMID:2547247, PMID:2550933, PMID:38355798
• Activation of the oxidase involves phosphorylation-dependent binding of NCF1 to the C-terminus of CYBA/p22-phox, promoting translocation of cytosolic subunits to the membrane (PubMed:12732142, PubMed:19801500). Related references: PMID:12732142, PMID:19801500
• The protein contains SH3 and PX domains, which are involved in protein-protein and lipid interactions, facilitating complex assembly.
• Subcellular localization includes cytoplasm, cytosol, and membrane; it is primarily cytosolic and translocates to the membrane upon activation.
• Tissue specificity: Detected in peripheral blood monocytes and neutrophils at the protein level.
• Keywords highlight its association with chronic granulomatous disease (CGD), alternative splicing, phosphoprotein, and SH3 domain.
• It plays a role in host defense, with mutations linked to CGD, an immunodeficiency characterized by recurrent infections.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-390, covering the entire protein sequence. This may enable detection of multiple isoforms if present.
• For Western blot, the expected molecular weight is approximately 45 kDa; consider verifying in relevant lysates from human, mouse, or rat monocytes/neutrophils.
• For immunofluorescence, permeabilization may be required to detect cytosolic p47phox; validate signal in activated vs. resting phagocytes.
• In immunoprecipitation, the antibody may co-precipitate other NADPH oxidase complex components; confirm with appropriate controls.
• For ELISA, test the antibody as capture or detection reagent in a sandwich assay; optimize concentrations and validate with known standards.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For projects targeting p47phox/NCF1, the central organizer of the phagocyte NADPH oxidase complex linked to chronic granulomatous disease, we offer validated polyclonal and monoclonal antibodies, enzyme conjugates, and ancillary reagents. Whether you need bulk quantities or rare target custom development, CamelBio bridges the gap between discovery and diagnostic application.