Anti-p38 MAPK (KO Validated) Rabbit Monoclonal Antibody for WB, ELISA - Q16539

Core Product Specifications and Parameters

Biological Background: p38 MAPK (MAPK14) Function and Localization

• MAPK14 is a serine/threonine kinase and a member of the p38 MAP kinase family. It is also known as p38 alpha, CSBP, SAPK2A, and has isoforms MXI2 and EXIP. The protein undergoes phosphorylation and acetylation and is involved in stress responses, inflammation, apoptosis, and transcriptional regulation.

• As a core component of the MAP kinase pathway, p38 MAPK is activated by extracellular stimuli such as pro-inflammatory cytokines and physical stress. It is estimated to phosphorylate 200–300 substrates, orchestrating broad cellular responses.

• Downstream kinases MSK1 (RPS6KA5) and MSK2 (RPS6KA4) are directly activated by p38 MAPK and phosphorylate transcription factors CREB1, ATF1, NF-kappa-B RELA, STAT1/3, as well as histone H3 and HMGN1, facilitating rapid immediate-early gene induction and chromatin remodeling. Related references: PMID:9687510 PMID:9792677

• Regulates gene expression post-transcriptionally via MK2 (MAPKAPK2) and MK3 (MAPKAPK3), which phosphorylate mRNA-binding proteins like ZFP36 and ELAVL1, and controls translation through MNK1/2-mediated phosphorylation of EIF4E2. Additionally, it interacts with casein kinase II to promote TP53 phosphorylation. Related references: PMID:11154262 PMID:10747897

• p38 MAPK modulates receptor endocytosis (e.g., clathrin-mediated EGFR internalization via phosphorylation of EGFR and RAB5A effectors) and ectodomain shedding by activating the metalloprotease ADAM17, linking inflammatory signals to growth factor signaling. Related references: PMID:16932740 PMID:20188673

• In the nucleus, it directly phosphorylates transcription factors ATF1, ATF2, ATF6, ELK1, DDIT3, TP53, MEF2C, and MEF2A, and enhances NF-kappa-B recruitment at inflammatory gene promoters by inducing histone H3 Ser10 phosphorylation. Related references: PMID:10330143 PMID:9430721 PMID:9858528

• Phosphorylates CDC25B/C leading to a G2 cell cycle delay after UV radiation; targets TIAR to prevent GADD45A mRNA degradation; and activates the NLRP1 inflammasome via phosphorylation, promoting pyroptosis. Related references: PMID:11333986 PMID:20932473 PMID:35857590

• Expressed in brain, heart, placenta, pancreas, and skeletal muscle; also plays an essential role in erythropoiesis by regulating EPO gene expression. During microbial infection, phosphorylation by M. tuberculosis EsxA inhibits IFN-gamma production in T-cells. Related references: PMID:10943842 PMID:21586573

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to residues 100-200 of human p38 MAPK (Q16539), a region conserved in mouse and rat, supporting the observed cross-reactivity. For Western blot, a band at approximately 41 kDa is anticipated. Titrate the antibody and include appropriate positive controls (e.g., cells stimulated with anisomycin or osmotic stress) to confirm reactivity.
• The antibody is KO validated, which typically ensures target specificity; however, every experimental system is unique, so validate using knockout or siRNA knockdown samples as appropriate.
• For ELISA, consider coating with purified recombinant p38 MAPK or cell lysates from stimulated cells. Assay conditions may require optimization of coating concentration, blocking, and detection steps.
• As a rabbit monoclonal antibody, it is generally suited for robust, reproducible results. Pair with a suitable anti-rabbit secondary antibody conjugate for detection in your protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For projects involving MAPK signaling, inflammation, or stress response biomarkers, this anti-p38 MAPK antibody is available alongside validated antibody pairs, bulk ancillary reagents, and rare target sourcing services. Contact our team to explore how CamelBio can streamline your raw material procurement and technical development needs.