Anti-P2Y12 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - Q9CPV9

Core Product Specifications and Parameters

Biological Background: P2Y12 Function and Localization

• P2Y12 is a purinergic G protein-coupled receptor (GPCR) for adenosine diphosphate (ADP) and adenosine triphosphate (ATP).
• It inhibits adenylyl cyclase through Gi protein coupling, reducing intracellular cyclic AMP levels.
• P2Y12 is essential for normal platelet aggregation and blood coagulation, playing a central role in hemostasis.
• Subcellularly, it localizes to the plasma membrane with seven transmembrane helices.
• The receptor undergoes post-translational modifications including glycosylation and phosphorylation, and contains disulfide bonds important for structure.
• No functionally characterized isoforms have been reported for the mouse P2ry12 gene.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fragment spanning amino acids 120–347, covers a region that includes both intracellular and extracellular domains, potentially enabling recognition of the native receptor.
• In Western blotting, the expected band is approximately 39 kDa (calculated molecular mass ~39.4 kDa); use platelet or megakaryocyte lysates as positive controls.
• For immunofluorescence, include a permeabilization step to access intracellular epitopes; validate staining pattern in mouse platelets or cultured cells expressing P2Y12.
• In ELISA, the antibody may be used as a capture or detection reagent; optimize using recombinant P2Y12 protein or platelet-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing this P2Y12 rabbit monoclonal antibody along with validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials. Whether your focus is on hemostasis research or developing diagnostic assays for platelet function, our one-stop supply chain supports your project with reliable materials and technical consultation.